- Clone
- HM48-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BCM1, SLAMF2, Blast-1
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HM48-1_Purified_CD48_Cytof_060414.jpg HM48-1_Purified_CD48_Cytof_060414.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HM48-1_Purified_CD48_Cytof_060414.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220 (RA36B2) and 154Sm-anti-CD48 (HM48-1). Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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103433 | 100 µg | $129 |
CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T lymphoma MBL-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
- Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
- Product Citations
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- RRID
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AB_2563753 (BioLegend Cat. No. 103433)
Antigen Details
- Structure
- Ig superfamily, 45 kD
- Distribution
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T cells and B cells, monocytes/macrophages
- Function
- Adhesion, T cell recognition
- Ligand/Receptor
- CD2, CD244
- Cell Type
- B cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809. - Gene ID
- 12506 View all products for this Gene ID
- UniProt
- View information about CD48 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD48 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 FITC -
PE anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 PE -
Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with purified HM48-1, foll... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Biotin anti-mouse CD48
C57BL/6 mouse splenocytes stained with biotinylated HM48-1, ... -
APC anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 APC -
Alexa Fluor® 488 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Alexa Fluor® 647 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Pacific Blue™ anti-mouse CD48
C57BL/6 mouse splenocytes stained with Pacific blue™ H... -
PE/Cyanine5 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine5 -
PerCP/Cyanine5.5 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Cyanine7 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700 -
Brilliant Violet 421™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Cyanine7 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Purified anti-mouse CD48 (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
Brilliant Violet 605™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 510™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 711™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Dazzle™ 594 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Fire™ 750 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
TotalSeq™-A0429 anti-mouse CD48
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Brilliant Violet 785™ anti-mouse CD48
C57BL/6 splenocytes were stained with CD48 (clone HM48-1) Br... -
Ultra-LEAF™ Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
TotalSeq™-B0429 anti-mouse CD48
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TotalSeq™-C0429 anti-mouse CD48
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