- Clone
- TER-119 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-76
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TER-119_Purified_TER-119_Cytof_061114.jpg TER-119_Purified_TER-119_Cytof_061114.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TER-119_Purified_TER-119_Cytof_061114.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes mixed with red blood cells and stained with 147Sm-anti-CD45 (30-F11) and 162Dy-anti-TER-119 (TER-119). Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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116241 | 100 µg | $118 |
The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Day-14 fetal liver cells from a C57BL/6 mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
- Vannucchi AM, et al. 2000. Blood 95:2559.
- Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
- Grisendi S, et al. 2005. Nature 437:147. (FC)
- Bourdeau A, et al. 2007. Blood 109:4220.
- Chappaz S, et al. 2007. Blood 110:3862. (FC)
- Heuser M, et al. 2007. Blood 110:1639. (FC)
- Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
- Product Citations
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- RRID
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AB_2563789 (BioLegend Cat. No. 116241)
Antigen Details
- Structure
- Associated with glycophorin A, 52 kD
- Distribution
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Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
- Cell Type
- Erythrocytes
- Biology Area
- Immunology
- Antigen References
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1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5. - Gene ID
- 104231 View all products for this Gene ID
- UniProt
- View information about TER-119 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All TER-119 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with TER-119 APC and anti-... -
Biotin anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with biotinylated TER... -
FITC anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with TER-119 FITC and anti... -
PE anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with TER-119 PE and anti-m... -
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells
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Purified anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with TER-119 FITC and CD45... Fresh, frozen mouse spleen was stained with purified TER-119... -
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells
C57BL/6 mouse bone marrow cells stained with TER119 Alexa Fl... -
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with TER-119 Alexa Fluor® ... -
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow stained cell with TER-119 Alexa Fluor&re... -
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells
C57BL/6 mouse bone marrow cells stained with TER119 PE/Cyani... -
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyan... -
PerCP anti-mouse TER-119/Erythroid Cells
C57BL/6 mouse bone marrow cells stained with TER-119 PerCP -
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells
C57BL/6 mouse bone marrow cells stained with TER-119 PerCP/C... -
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells
C57BL/6 splenocytes mixed with red blood cells were stained ... -
Pacific Blue™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells stained with Ter-119 Pacific Blue&... -
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 PE and TER-... -
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 Pacific Blu... -
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 FITC and TE... -
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)
C57BL/6 mouse splenocytes mixed with red blood cells and sta... -
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 APC and TER... -
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 FITC and TE... -
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells
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APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with CD45 FITC and TE... -
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells
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TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells
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Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells
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Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells
C57BL/6 mouse bone marrow cells stained with CD45 PE and Ter... -
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow were stained with CD45 PE and TER-119 (c... -
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells
C57BL/6 bone marrow cells were stained with anti-mouse CD45 ...
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