- Clone
- 11G10B39 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![11G10B39_PURE_DDX6_Antibody_1_WB_121217 11G10B39_PURE_DDX6_Antibody_1_WB_121217](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/11G10B39_PURE_DDX6_Antibody_1_WB_121217.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell lysates (15 µg protein) from Jurkat, HeLa, and NIH/3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-DDX6 antibody, clone 11G10B39. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot HRP anti-β-actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder. -
Whole cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with the indicated concentration of anti-DDX6 antibody, clone 11G10B39. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot HRP anti-β-actin antibody (1:5000 dilution, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder. -
A431 cells were stained with purified anti-DDX6 clone (11G10B39) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
674402 | 100 µg | $288 |
DDX6, also known as p54/RCK, is a member of the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase family. DDX6 has been shown to localize to, and is required for the assembly of, processing bodies (P-bodies), which are cytoplasmic foci that consist of proteins involved in mRNA storage, processing, and turnover. DDX6 has ATP hydrolysis and RNA helicase activities, which are involved in secondary structure modulation and the degradation of mRNA. Interacting with key components of miRNA-induced silencing complexes, DDX6 participates in translational repression, as well. DDX6 is overexpressed in some colorectal tumor cells and can act as a proto-oncogene.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human DDX6 recombinant protein (1-126 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.25 µg/mL (1:2000-1:5000 dilution). For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
At high antibody concentrations, a weak, non-specific band of ~30 kDa may appear in western blots.
- RRID
-
AB_2565215 (BioLegend Cat. No. 674402)
Antigen Details
- Structure
- 483 amino acids with a predicted molecular weight of 54 kD and contains a DEAD box motif and a C-terminal helicase domain.
- Distribution
-
Cytoplasm and P-bodies.
- Function
- DDX6 is a RNA-helicase with RNA-dependent ATP hydrolysis and ATP-dependent RNA unwinding activities that mediates mRNA translation, processing, and turnover.
- Interaction
- Interacts with LIMD1, WTIP, AJUBA, RC3H1, APOEBC3, AGO1, AGO2, and forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
- Biology Area
- Cell Biology
- Antigen References
-
1. Pettersson OJ, et al. 2014. Nucleic Acids Res. 42:7186.
2. Huys A, et al. 2013. PLoS One 8:e67437.
3. Reed JC, et al. 2012. J. Cell Biol. 198:439.
4. Yu SF, et al. 2011. PLoS Pathog. 7:e1002303.
5. Bidet K, et al. 2011. RNA Biol. 6:1173.
6. Jangra RK, et al. 2010. J. Virol. 84:6810. - Gene ID
- 1656 View all products for this Gene ID
- UniProt
- View information about DDX6 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All DDX6 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-DDX6 | 11G10B39 | WB,IP,ICC |
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