- Clone
- HM48-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BCM1, SLAMF2, Blast-1
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HM48-1_BV605_CD48_Antibody_013017 HM48-1_BV605_CD48_Antibody_013017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HM48-1_BV605_CD48_Antibody_013017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) Brilliant Violet 605™ (filled histogram) or Armenian hamster IgG Brilliant Violet 605™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
103441 | 50 µg | $309 |
CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T lymphoma MBL-2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
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Application References
(PubMed link indicates BioLegend citation) -
- Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
- Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
- Product Citations
-
- RRID
-
AB_2650825 (BioLegend Cat. No. 103441)
Antigen Details
- Structure
- Ig superfamily, 45 kD
- Distribution
-
T cells and B cells, monocytes/macrophages
- Function
- Adhesion, T cell recognition
- Ligand/Receptor
- CD2, CD244
- Cell Type
- B cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809. - Gene ID
- 12506 View all products for this Gene ID
- UniProt
- View information about CD48 on UniProt.org
Other Formats
View All CD48 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 FITC -
PE anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 PE -
Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with purified HM48-1, foll... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Biotin anti-mouse CD48
C57BL/6 mouse splenocytes stained with biotinylated HM48-1, ... -
APC anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 APC -
Alexa Fluor® 488 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Alexa Fluor® 647 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor&re... -
Pacific Blue™ anti-mouse CD48
C57BL/6 mouse splenocytes stained with Pacific blue™ H... -
PE/Cyanine5 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine5 -
PerCP/Cyanine5.5 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Cyanine7 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700 -
Brilliant Violet 421™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Cyanine7 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Purified anti-mouse CD48 (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
Brilliant Violet 605™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 510™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
Brilliant Violet 711™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
PE/Dazzle™ 594 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
APC/Fire™ 750 anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48... -
TotalSeq™-A0429 anti-mouse CD48
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Brilliant Violet 785™ anti-mouse CD48
C57BL/6 splenocytes were stained with CD48 (clone HM48-1) Br... -
Ultra-LEAF™ Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
TotalSeq™-B0429 anti-mouse CD48
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TotalSeq™-C0429 anti-mouse CD48
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