- Clone
- SMI 25 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glial fibrillary acidic protein
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![IHC staining of HRP anti-GFAP Antibody (Clone SMI 25) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 5 µg/mL for one hour at room temperature. DAB was used for detection followed by hematoxylin and Bluing solution counterstaining. IHC staining of HRP anti-GFAP Antibody (Clone SMI 25) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 5 µg/mL for one hour at room temperature. DAB was used for detection followed by hematoxylin and Bluing solution counterstaining.](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SMI-25_HRP_GFAP_Antibody_WB_072417.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of HRP anti-GFAP (clone SMI 25), and isotype-matched IgG2b control. Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. Blots were incubated with 1 µg/mL of each primary antibody overnight at 4°C. The isotype-matched blot was further incubated with secondary antibody. Enhanced chemiluminescence was used as the detection system. -
IHC staining of HRP anti-GFAP Antibody (Clone SMI 25) on formalin-fixed paraffin-embedded human cerebellum brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 5 µg/mL for one hour at room temperature. DAB was used for detection followed by hematoxylin and Bluing solution counterstaining.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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837505 | 25 µg | $124 |
Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.
Type III intermediate filaments are highly conserved and contain three domains, named the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments: in cells that express both proteins, two separate intermediate filament networks form.
To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated to HRP under optimal conditions
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg per ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2721603 (BioLegend Cat. No. 837505)
Antigen Details
- Structure
- GFAP is a 432 amino acid protein with a molecular mass of approximately 50 kD.
- Distribution
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Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: cytoskeleton and cytosol - Function
- GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Khakh BS, Sofroniew MV. 2015. Nat. Neurosci. 18:942-52. PubMed
- Gene ID
- 2670 View all products for this Gene ID
- UniProt
- View information about GFAP on UniProt.org
Other Formats
View All GFAP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-GFAP | SMI 25 | IHC-P,WB |
Purified anti-GFAP | SMI 25 | IHC-P,WB |
HRP anti-GFAP | SMI 25 | IHC-P,WB |
Alexa Fluor® 594 anti-GFAP | SMI 25 | IHC-P |
Alexa Fluor® 488 anti-GFAP | SMI 25 | IHC-P |
Alexa Fluor® 647 anti-GFAP | SMI 25 | IHC-P,SB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-GFAP
IHC staining of anti-GFAP antibody (clone SMI 25) on formali... Western blot of anti-GFAP antibody (clone SMI 25). Lane 1: M... -
Purified anti-GFAP
IHC staining of purified anti-GFAP antibody (clone SMI 25) o... Western blot of purified anti-GFAP antibody (clone SMI 25). ... -
HRP anti-GFAP
IHC staining of HRP anti-GFAP Antibody (Clone SMI 25) on for... Western blot of HRP anti-GFAP (clone SMI 25), and isotype-ma... -
Alexa Fluor® 594 anti-GFAP
IHC staining of Alexa Fluor® 594 anti-GFAP antibody (clone S... -
Alexa Fluor® 488 anti-GFAP
IHC staining of Alexa Fluor® 488 anti-GFAP antibody (clone S... IHC staining of Alexa Fluor® 594 anti-Rat Blood-Brain Barrie... IHC staining of Alexa Fluor® 488 anti-GFAP antibody (clone S... -
Alexa Fluor® 647 anti-GFAP
IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone S... IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone S... IHC staining of Alexa Fluor® 647 anti-GFAP antibody (clone S...
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