- Clone
- ME-9F1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_ME-9F1_A594_CD146_Antibody_IHCP_040318 A_ME-9F1_A594_CD146_Antibody_IHCP_040318](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/ME-9F1_A594_CD146_Antibody_IHCP_original_040318.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD146 (clone ME-9F1) Alexa Fluor® 594 (red), anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (green), and anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective. -
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was stained with anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 594 at 5 µg/mL (yellow) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # | Size | Price | Quantity Check Availability | Save | ||
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134719 | 25 µg | $153 | ||||
134720 | 100 µg | $305 |
CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Endothelial cell line TME-3H3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
3D IHC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses -
Application References
(PubMed link indicates BioLegend citation) -
- Schrage A, et al. 2008. Histochem. Cell. Biol. 129:441.
- Birbrair A, et al. 2013. Am J Physiol Cell Physiol. 305:1098. PubMed
- RRID
-
AB_2728156 (BioLegend Cat. No. 134719)
AB_2728157 (BioLegend Cat. No. 134720)
Antigen Details
- Structure
- CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
- Distribution
-
CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
- Function
- CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
- Ligand/Receptor
- Unidentified ligand.
- Cell Type
- Endothelial cells, Mesenchymal Stem Cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Despoix N, et al. 2008. Eur. J. Immunol. 38:2855.
2. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
3. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746. - Gene ID
- 84004 View all products for this Gene ID
- UniProt
- View information about CD146 on UniProt.org
Related FAQs
Other Formats
View All CD146 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PerCP/Cyanine5.5 anti-mouse CD146 | ME-9F1 | FC |
Purified anti-mouse CD146 | ME-9F1 | FC,IHC-F |
PE anti-mouse CD146 | ME-9F1 | FC |
FITC anti-mouse CD146 | ME-9F1 | FC |
Alexa Fluor® 488 anti-mouse CD146 | ME-9F1 | FC,IHC-F |
APC anti-mouse CD146 | ME-9F1 | FC |
PE/Cyanine7 anti-mouse CD146 | ME-9F1 | FC |
Alexa Fluor® 647 anti-mouse CD146 | ME-9F1 | FC,IHC-F,3D IHC |
Biotin anti-mouse CD146 | ME-9F1 | FC |
Alexa Fluor® 594 anti-mouse CD146 | ME-9F1 | IHC-F,3D IHC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PerCP/Cyanine5.5 anti-mouse CD146
bEND.3 cells (mouse endothelial cells) were stained with CD1... -
Purified anti-mouse CD146
C57BL/6 splenocytes stained with ME-9F1 PE and NK-1.1 (PK136... C57BL/6 splenocytes stained with rat IgG2a (RTK2758) PE isot... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PE anti-mouse CD146
C57BL/6 splenocytes stained with ME-9F1 PE and NK-1.1 (PK136... C57BL/6 splenocytes stained with rat IgG2a (RTK2758) PE isot... -
FITC anti-mouse CD146
bEND.3 cells (mouse endothelial cells) stained with ME-9F1 F... -
Alexa Fluor® 488 anti-mouse CD146
C57BL/6 splenocytes stained with anti-mouse NK-1.1 (PK136) A... C57BL/6 splenocytes stained with NK-1.1 (PK136) APC and rat ... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
APC anti-mouse CD146
Mouse endothelial cells were stained with CD146 (clone ME-9F... -
PE/Cyanine7 anti-mouse CD146
bEnd.3, mouse endothelial cells were stained with CD146 (clo... -
Alexa Fluor® 647 anti-mouse CD146
bEnd.3 cells (mouse endothelial cells) were stained with CD1... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
Biotin anti-mouse CD146
bEND.3 cells (mouse endothelial cells) were stained with bio... -
Alexa Fluor® 594 anti-mouse CD146
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti...
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