- Clone
- 4B12-E11-D9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Phosphatase 1 Regulatory Subunit 18, Protein Phosphatase 1 F-Actin Cytoskeleton Targeting Subunit, KIAA1949
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4B12-E11-D9_Purified_Phostensin_Antibody_1_050919.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Phostensin antibody (4B12-E11-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cytoskeleton lysate. The blot was incubated with 2.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Phostensin antibody (4B12-E11-D9) on formalin-fixed paraffin-embedded human lymph node tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
865801 | 25 µg | $106 | ||||
865802 | 100 µg | $276 |
Phostensin is a protein that targets protein phosphatase 1 (PP1) to F-actin cytoskeleton. The phostensin/PP1 complex is involved in actin cytoskeleton remodeling. Diseases associated with phostensin include deafness and autosomal recessive 79.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human phostensin protein fragments expressed in E.coli
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2810781 (BioLegend Cat. No. 865801)
AB_2810782 (BioLegend Cat. No. 865802)
Antigen Details
- Structure
- Phostensin is a 613 amino acid protein with an observed molecular mass of ~100 kD.
- Distribution
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Tissue Distribution: Brain, spleen, lymph node, and leukocytes
Cellular Distribution: Cytoskeleton, nucleus, and cytosol - Function
- Phostensin is involved in the regulation of protein phosphase-1 activity by targeting it to the F-actin cytoskeleton.
- Interaction
- Protein Phosphatase-1, F-actin
- Cell Type
- Lymphocytes, Neurons
- Biology Area
- Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction
- Molecular Family
- Cytoskeletal Proteins, Enzymes and Regulators, Phospho-Proteins, Protein Modifiers
- Antigen References
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- Matsubara T, et al. 2018. Mol Cell Biol. 38(4). pii: e00425.
- Lin YS, et al. 2011. J Histochem Cytochem. 59(8):741.
- Gene ID
- 170954 View all products for this Gene ID
- UniProt
- View information about Phostensin on UniProt.org
Related FAQs
Other Formats
View All Phostensin Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-Phostensin | 4B12-E11-D9 | WB,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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