- Clone
- A18072A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NRAS Proto-Oncogene, GTPase NRas, Neuroblastoma RAS Viral Oncogene Homolog
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A18072A_PURE_NRAS_Antibody_1_061819 A18072A_PURE_NRAS_Antibody_1_061819](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18072A_PURE_NRAS_Antibody_1_061819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-NRAS antibody (clone A18072A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-NRAS antibody (clone A18072A) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-NRAS antibody (clone A18072) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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866101 | 25 µg | $106 | ||||
866102 | 100 µg | $276 |
NRAS is a Ras gene family member originally identified in human neuroblastoma cells. Like other members of the Ras family, NRAS has GTP/GDP binding and GTPase activity, and functions as a G-like regulatory protein that plays a role in regulating cell growth. Mutations in NRAS have been shown to transform cultured cells to tumor cells, implicating its role in tumorigeneses, as potential therapeutic target for cancers such as melanoma.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human NRAS a.a. 1-186
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2810783 (BioLegend Cat. No. 866101)
AB_2810784 (BioLegend Cat. No. 866102)
Antigen Details
- Structure
- NRAS is a 189 amino acid protein with predicted and observed molecular mass of ~ 21 kD.
- Distribution
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Tissue Distribution: Ubiquitously expressed. Highly expressed in appendix, gallbladder, and gastrointestinal tract.
Cellular Distribution: Plasma membrane, Golgi apparatus, cytosol, and nucleus. - Function
- G-Like regulatory protein that plays a role in regulating cell growth.
- Interaction
- RGS14, RASSF7
- Ligand/Receptor
- GTP, GDP
- Cell Type
- Astrocytes, Endothelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Oligodendrocytes
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neuroscience, Stem Cells
- Molecular Family
- GTPases
- Antigen References
-
- Rocks O, et al. 2005. Science. 307:1746-52.
- Byrne M, Saif MW. 2019. Onco. Targets Ther. S194605
- Sexton RE, et al. 2019. Semin. Cancer Biol. 54:131-7.
- Gene ID
- 4893 View all products for this Gene ID 24605 View all products for this Gene ID
- UniProt
- View information about NRAS on UniProt.org
Related FAQs
Other Formats
View All NRAS Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-NRAS | A18072A | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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