- Clone
- A18059A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Galectin 7, Gal-7, GAL7, HKL-14, PI7, PIG1, p53-induced gene 1 protein, TP53I1, LGALS7A
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A18059A_HRP_Galectin-7_Antibody_1_070220 A18059A_HRP_Galectin-7_Antibody_1_070220](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18059A_HRP_Galectin-7_Antibody_1_070220.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of HRP anti-Galectin-7 antibody (clone A18059A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human skin lysate; Lane 3: 20 µg of mouse skin lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. -
IHC staining of HRP anti-Galectin-7 antibody (clone A18059A) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-Galectin-7 antibody (clone A18059A) on formalin-fixed paraffin-embedded mouse skin tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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866309 | 25 µg | $141 |
The galectins are a family of beta-galactoside-binding proteins implicated in cell-cell and cell-matrix interactions. Galectin-7 is specifically expressed in stratified epithelia and epidermis, but barely detected in epidermal tumors and absent from squamous carcinoma cell lines. Galectin-7 is a p53-induced protein and is abnormally expressed in variety of tumors such as melanoma, lung cancer and colon cancer. In UVB-induced sunburn keratinocytes, the expression of Galectin-7 is markedly increased due to the genotoxic stress-induced p53 activation. Both positive and negative regulatory roles of Galectin-7 have been reported in tumorigenesis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human Galectin-7
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.5 - 10 µg/mL.For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2876749 (BioLegend Cat. No. 866309)
Antigen Details
- Structure
- Human Galectin-7 is a 136 amino acid protein with a molecular mass of 15 kD.
- Distribution
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Tissue distribution: Stratified squamous epithelium
Cellular distribution: Nucleus, extracellular region or secreted, and cytoplasm - Function
- Galectin-7 is involved in the cell-cell and cell-matrix interactions.
- Ligand/Receptor
- Carbohydrates
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Cell Adhesion, Cell Biology, Neurodegeneration
- Molecular Family
- Adhesion Molecules, Soluble Receptors
- Antigen References
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- Bernerd F. et al. 1999. Proc Natl Acad Sci U S A. 96(20):11329-34.
- Madsen P. et al. 1995. J Biol Chem. 270(11):5823-9.
- Biron-Pain K. et al. 2013. PLoS One. 8(5):e63307.
- Gene ID
- 3963 View all products for this Gene ID
- UniProt
- View information about Galectin-7 on UniProt.org
Related FAQs
Other Formats
View All Galectin-7 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Galectin-7 | A18059A | WB,IHC-P |
Alexa Fluor® 647 anti-Galectin-7 | A18059A | IHC-P |
Alexa Fluor® 594 anti-Galectin-7 | A18059A | IHC-P |
Alexa Fluor® 488 anti-Galectin-7 | A18059A | IHC-P |
HRP anti-Galectin-7 | A18059A | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Galectin-7
Western blot of purified anti-Galectin-7 antibody (clone A18... IHC staining of purified anti-Galectin-7 antibody (clone A18... IHC staining of purified anti-Galectin-7 antibody (clone A18... -
Alexa Fluor® 647 anti-Galectin-7
IHC staining of Alexa Fluor® 647 anti-Galectin-7 antibody (c... IHC staining of Alexa Fluor® 647 anti-Galectin-7 antibody (c... -
Alexa Fluor® 594 anti-Galectin-7
IHC staining of Alexa Fluor® 594 anti-Galectin-7 antibody (c... IHC staining of Alexa Fluor® 594 anti-Galectin-7 antibody (c... -
Alexa Fluor® 488 anti-Galectin-7
IHC staining of Alexa Fluor® 488 anti-Galectin-7 antibody (c... IHC staining of Alexa Fluor® 488 anti-Galectin-7 antibody (c... -
HRP anti-Galectin-7
Western blot of HRP anti-Galectin-7 antibody (clone A18059A)... IHC staining of HRP anti-Galectin-7 antibody (clone A18059A)... IHC staining of HRP anti-Galectin-7 antibody (clone A18059A)...
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