- Clone
- N377/20 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitogen-Activated Protein Kinase Kinase Kinase 12, DLK, Dual Leucine Zipper Kinase DLK, MAPK-Upstream Kinase, Mixed Lineage Kinase, Leucine Zipper Protein Kinase, MEKK12
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N37720_PURE_MAP3K12_Antibody_110921 N37720_PURE_MAP3K12_Antibody_110921](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N37720_PURE_MAP3K12_Antibody_110921.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole tissue membrane extracts (15 µg total protein) from rat and mouse brain were heated at 37°C for 30 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-MAP3K12 antibody (clone N377/20) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
Cat # | Size | Price | Quantity Check Availability | Save | ||
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608252 | 100 µg | $165 |
Mitogen-activated protein kinase kinase kinase 12 (MAP3K12), also known as dual leucine zipper kinase (DLK), is a serine/threonine-protein kinase and part of the non-canonical MAPK signaling pathway. Primarily expressed in neurons at the synaptic terminals, MAP3K12 is a critical regulator of signaling cascades affecting neuronal migration and axon development as well as responding to peripheral nerve injury including transcriptional repression, axon regeneration, and neuronal apoptosis through its activation of transcription factors STAT3 and c-Jun. Its neuroprotective role in responding to injury makes MAP3K12 a promising therapeutic target in the study of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis (ALS), and optic neuropathy. Emerging evidence suggests a role for MAP3K12 in the proliferation and mass regulation of pancreatic beta cells via activation of c-Jun N-terminal kinase (JNK) signaling pathway and may be a target of interest in the study of the pathogenesis of insulin resistance.
Product DetailsProduct Details
- Verified Reactivity
- Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein corresponding to amino acids 727-888 (C-terminus) of mouse MAP3K12
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone has been tested for IHC and failed.
- RRID
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AB_2904449 (BioLegend Cat. No. 608252)
Antigen Details
- Structure
- MAP3K12 is an 859 amino acid protein with a predicted molecular weight of 93 kD.
- Distribution
-
Ubiquitously expressed in cell membrane and cytoplasm
- Function
- Serine/threonine-protein kinase, transferase
- Cell Type
- Mature Neurons
- Biology Area
- Neuroscience, Neuroscience Cell Markers
- Antigen References
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- Huntwork-Rodriguez S, et al. 2013. J. Cell Biol. 202:747.
- Patel S, et al. 2014. J. Med. Chem. 58:401.
- Shin J, et al. 2019. Neurobio. Dis. 127:178.
- Tenenbaum M, et al. 2021. Cell. Mol. Life Sci. 78:287.
- Gene ID
- 7786 View all products for this Gene ID
- UniProt
- View information about MAP3K12 on UniProt.org
Related FAQs
Other Formats
View All MAP3K12 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MAP3K12 | N377/20 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-MAP3K12
Whole tissue membrane extracts (15 µg total protein) from ra...
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