- Clone
- 27-9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Junctional adhesion molecule-1,Jam-1, Jam-A, junctional adhesion molecule-A, CD321
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![27-9_PURE_CD321_Antibody_1_112221 27-9_PURE_CD321_Antibody_1_112221](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/27-9_PURE_CD321_Antibody_1_112221.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 platelets were stained with anti-mouse CD41 APC and purified anti-mouse CD321 (F11R) (clone 27-9) purified (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE. -
C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. The section was stained with 5 µg/mL with purified anti-mouse CD321 (F11R) (clone 27-9) followed by anti-mouse IgG Alexa Fluor® 555. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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107802 | 100 µg | $300 |
F11R is a cell adhesion molecule and immunoglobulin superfamily member and an important regulator of tight junction assembly in epithelia. It is found on the surface of platelets, and plays a role in platelet aggregation, secretion, adhesion, and spreading. By interacting with integrin alpha-L/beta-2, it is involved in leukocyte transmigration.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E14.5 mouse fetal liver cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.5 µg per million cells in 100 µL volume. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Sugano Y et al. 2008. Blood. 111(3): 1167–72.
- RRID
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AB_2904280 (BioLegend Cat. No. 107802)
Antigen Details
- Structure
- 32 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
- Distribution
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Cell membrane, tight junctions
- Function
- Protein homodimerization, integrin binding, PDZ domain binding, tight junction formation
- Interaction
- integrin alpha-L/beta-2, MPDZ, PARD3, ITGAL
- Ligand/Receptor
- integrin alpha-L/beta-2
- Cell Type
- Endothelial cells, Epithelial cells, Leukocytes, Platelets
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Martin-Padura I, et al. 1998. J. Cell Biol. 142:117-27.
- Muller W.A. 2003. Trends Immunol. 24:327-34.
- Kostrewa D., et al. 2001. EMBO J. 20:4391-8.
- Gene ID
- O88792 View all products for this Gene ID
- UniProt
- View information about CD321 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD321 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD321 (F11R) | 27-9 | FC,IHC-F |
PE anti-mouse CD321 (F11R) | 27-9 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD321 (F11R)
C57BL/6 platelets were stained with anti-mouse CD41 APC and ... C57BL/6 mouse frozen kidney section was fixed with 4% parafo... -
PE anti-mouse CD321 (F11R)
C57BL/6 platelets (whole blood) were stained with anti-mouse...
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