- Clone
- QA19A52 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD45R
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_QA19A52_PURE_CD45R_Antibody_1_042122 1_QA19A52_PURE_CD45R_Antibody_1_042122](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA19A52_PURE_CD45R_Antibody_1_042122.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD3 FITC and purified anti-mouse CD45R/B220 recombinant (clone QA19A52) (left) or rat IgG2a, κ isotype (right) followed by APC anti-rat IgG. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse CD45R/B220 recombinant (clone QA19A52) and Alexa Fluor® 594 anti-mouse CD3ε (clone 500A2) (green) overnight at 4°C. On the next day, sections were washed and stained with 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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111302 | 100 µg | $88 |
CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker. CD19, however, may be more appropriate for B cell specificity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2927819 (BioLegend Cat. No. 111302)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
B cells, T cell subset, NK cell subset
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Trowbridge IS, et al. 1994. Annu Rev Immunol. 12:85-116.
- Kishihara K, et al. 1993. Cell 74:143-56.
- Pulido R, et al. 1988. J Immunol. 140:3851-7.
- Gene ID
- 19264 View all products for this Gene ID 5788 View all products for this Gene ID
- UniProt
- View information about CD45R on UniProt.org
Related FAQs
Other Formats
View All CD45R/B220 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD45R/B220 Recombinant Antibody | QA19A52 | FC,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD45R/B220 Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 F... C57BL/6 mouse frozen spleen section was fixed with 4% parafo...
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