Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- 4S.B3
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
- Isotype
- Mouse IgG1, κ
![4SB3_APC_IFN-gamma_ASR_020323 4SB3_APC_IFN-gamma_ASR_020323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4SB3_APC_IFN-gamma_ASR_020323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical result of PMA + Ionomycin with Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes fixed with Fixation Buffer (Cat# 420801), permeabilized with Permeabilization Wash Buffer (Cat# 421002), and intracellularly stained either with 4S.B3 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
986702 | 500 µL | $370 |
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 25 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
Suggested for Intracellular Staining for Flow Cytometry
- Disclaimer
-
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
Antigen Details
- Antigen References
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- Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
- De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
- Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
- Gray P, et al. 1987. Lymphokines. 13:151.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human IFN-γ
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 P... -
APC anti-human IFN-γ
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
FITC anti-human IFN-γ
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Biotin anti-human IFN-γ
-
Purified anti-human IFN-γ
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 P... Western blot analysis of 50 ng recombinant human IFN-γ (lane... -
Alexa Fluor® 488 anti-human IFN-γ
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Alexa Fluor® 647 anti-human IFN-γ
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Alexa Fluor® 700 anti-human IFN-γ
PMA+ionomycin-stimulated (5 hours) human PBMCs surface stain... -
Pacific Blue™ anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PerCP/Cyanine5.5 anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
APC/Cyanine7 anti-human IFN-γ
PMA + Ionomycin (6 hours) stimulated human PBMCs were surfac... -
PE/Cyanine7 anti-human IFN-γ
PMA + ionomycin-stimulated (6 hours, in the presence of Bref... -
Brilliant Violet 421™ anti-human IFN-γ
Human peripheral blood lymphocytes were stimulated with PMA ... -
Brilliant Violet 570™ anti-human IFN-γ
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 605™ anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 650™ anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood l... -
Brilliant Violet 711™ anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 785™ anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 510™ anti-human IFN-γ
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
PE/Dazzle™ 594 anti-human IFN-γ
PMA+ Ionomycin-human peripheral blood lymphocytes (in the pr... -
APC/Fire™ 750 anti-human IFN-γ
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
PerCP anti-human IFN-γ
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 750™ anti-human IFN-γ
PMA/ionomycin-stimulated (4 hours) human peripheral blood ly... -
KIRAVIA Blue 520™ anti-human IFN-γ Antibody
PMA + Ionomycin (6 hours) stimulated human PBMCs were surfac... -
Spark NIR™ 685 anti-human IFN-γ Antibody
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
APC anti-human IFN-γ
Typical result of PMA + Ionomycin with Brefeldin A stimulate... -
Spark Blue™ 515 anti-human IFN-γ
PMA+ Ionomycin (5 hours) stimulated human PBMCs were surface... -
Spark UV™ 387 anti-human IFN-γ
Human peripheral blood lymphocytes were stimulated with Cell... -
Spark PLUS UV™ 395 anti-human IFN-γ
Human peripheral blood lymphocytes were stimulated with PMA/... -
PE anti-human IFN-γ
Typical results from PMA + Ionomycin with Brefeldin A stimul...
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