- Clone
- A21018C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glycogen synthase kinase-3 beta; GSK3B, GSK-3B, GSK-3beta
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a. A21018C_-Purified_-GSK3b_WB_100523 a. A21018C_-Purified_-GSK3b_WB_100523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A21018C_-Purified_-GSK3b_WB_100523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from Jurkat cells untreated or treated with wortmannin were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-GSK3b Phospho (Ser9) (clone A21018C) (left), or anti- GSK3b Pan (right) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
Whole cell extracts (15 µg total protein) from NIH/3T3 cells untreated or treated with PDGF were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-GSK3b Phospho (Ser9) (clone A21018C) (left), or anti-GSK3b Pan (right) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
Jurkat cells untreated (positive control, filled histogram) or treated with wortmannin (negative control, open histogram) (left), or NIH/3T3 cells untreated (negative control, open histogram) or treated with PDGF-BB (positive control, filled histogram) (right) were fixed and permeabilized using True-Phos™ Fix/Perm Buffer Set (Cat. No. 425401). Then, cells were intracellularly stained with purified anti-GSK3β Phospho (Ser9) (clone A21018C), or purified mouse IgG1, κ isotype control (open histogram, dashed line) (Cat. No. 400101), followed by PE goat anti-mouse IgG (Cat. No. 405307).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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618551 | 25 µg | $165 | ||||
618552 | 100 µg | $420 |
Glycogen synthase kinase 3 beta or GSK3β, is a 420 aa length constitutively active serine-threonine kinase that phosphorylates more than 100 target proteins. GSK3β is involved in several biological activities including glycogen metabolism, cell signaling, cellular transport, cell proliferation, apoptosis, and others. The activity of GSK3β is regulated by phosphorylation at different residues. Phosphorylation at tyrosine 216 enhances GSK3β kinase activity, while phosphorylation at serine 9 decreases kinase activity. In addition, GSK3β activity can also be regulated by cellular localization and/or formation of protein complexes. GSK3β has been associated with several diseases including type 2 diabetes, Alzheimer’s disease, inflammation, cancer, addiction, and bipolar disorders.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human GSK3β phosphorylated at Serine 9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ICFC: Cells were fixed and permeabilized using the True-Phos™ Buffer system
- RRID
-
AB_3068059 (BioLegend Cat. No. 618551)
AB_3068059 (BioLegend Cat. No. 618552)
Antigen Details
- Structure
- GSK3β is a 420 a.a protein with a predicted molecular weight approximately 47 kD. GSK3β has a bi-lobar architecture, with N-terminal and C- terminal domains. The N-terminal is responsible for ATP binding, while the C-terminal carries the activation loop that mediates the kinase activity.
- Distribution
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GSK3β can be found mainly in the cytoplasm, however, it could also be present in mitochondria and nucleus.
- Function
- It is a serine/threonine Kinase, its activity can be enhanced by phosphorylation on its tyrosine 216, or reduced by phosphorylation on its serine 9.
- Interaction
- Glycogen synthase, Beta-catenin/Wnt, Insulin receptor, IRS1, MAPT/Tau proteins, NF-κB, MUC1, MACF1, E2F1, mTORC2, JUN, NFATC
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology
- Molecular Family
- Phospho-Proteins
- Antigen References
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- Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998.e5.
- Xing B, et al. 2018. Glia. 66:1999-2012.
- Ryu HY, et al. 2021. Exp Mol Med. 53:369-383.
- Wu H, et al. 2019. Autophagy. 15:1506-1522.
- Gene ID
- 2932 View all products for this Gene ID
- UniProt
- View information about GSK3b Phospho (Ser9) on UniProt.org
Related FAQs
Other Formats
View All GSK3b Phospho (Ser9) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GSK3β Phospho (Ser9) | A21018C | WB,ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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