- Clone
- W21127K (See other available formats)
- Regulatory Status
- RUO
- Other Names
- VCAM-1, INCAM-110
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A. W21127K_PURE_CD106_FC_090723 A. W21127K_PURE_CD106_FC_090723](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W21127K_PURE_CD106_FC_090723-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with anti-mouse/human CD11b APC and purified anti-mouse CD106 (clone W21127K) (left) or purified rat IgG2a, κ isotype control (right) followed by PE goat anti-rat IgG2a. -
IHC staining of Purified anti-mouse CD106 (Ep-CAM) (clone W21127K) on formalin-fixed paraffin-embedded C57BL/6 mouse testis tissue. Following antigen retrieval using 1X citrate buffer 1X (10 mM, pH 6.0) (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) 1 µg/mL of purified anti-mouse CD106 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (red) (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm -
IHC staining of purified anti-mouse CD106 (Ep-CAM) (clone W21127K) on frozen mouse testis tissue. C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated 5 μg/mL of purified anti-mouse CD147 and without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD106 (Ep-CAM) at 4°C overnight, followed by incubation 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG2a (red) (Cat. No. 407512) and Alexa Fluor® 594 anti-rat IgG1 (green) (Cat. No. 407410) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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117002 | 100 µg | $110 |
CD106, also known as VCAM-1 and INCAM-110, is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin).
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Transfectant cells (aa25-692) and recombinant protein (aa25-692)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P, IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Flow cytometry testing showed that clone W21127K does not affect the bindings of clones 429 (MVCAM.A) and W21127Q on target cells.
- RRID
-
AB_3097228 (BioLegend Cat. No. 117002)
Antigen Details
- Structure
- Ig superfamily, 47 kD
- Distribution
-
Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
- Function
- Adhesion
- Ligand/Receptor
- VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
- Cell Type
- Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Kinashi T, et al. 1995. J Leukoc Biol. 57:168-73.
- Bevilacquea MP. 1993. Annu Rev Immunol. 11:767-804.
- Koni PA, et al. 2001. J Exp Med. 193:741-54.
- Gene ID
- 22329 View all products for this Gene ID
- UniProt
- View information about CD106 on UniProt.org
Related FAQs
Other Formats
View All CD106 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD106 | W21127K | FC,IHC-P,IHC-F |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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