- Clone
- P84F5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GATA Binding Protein 1, Erythroid Transcription Factor, Globin Transcription Factor 1, NFE1, XLTT
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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P84F5_Alexa-Fluor-647_GATA1_ICFC_052924 A.
P84F5_Alexa-Fluor-647_GATA1_ICFC_052924](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P84F5_Alexa-Fluor-647_GATA1_ICFC_052924-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HEL cells (positive control, filled histogram) and HeLa cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-GATA1 (clone P84F5) or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400330). -
HEL cells were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with ice-cold methanol and then blocked with 5% FBS. Cells were then stained with Alexa Fluor® 647 mouse IgG2b, κ isotype control (Cat. No. 400330) (panel A) or Alexa Fluor® 647 anti-GATA1 (clone P84F5) (panel B) overnight. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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939105 | 25 µg | $180 | ||||
939106 | 100 µg | $450 |
The GATA family of transcription factors is comprised of six members characterized by their binding to the consensus DNA sequence (T/A)GATA(A/G). GATA1, which was the initially described member of the GATA family, is predominantly expressed in cells of the myeloid lineage such as erythrocytes, eosinophils, mast cells, and megacaryocytes. Over the course of erythroid differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exchanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch. Genes induced by GATA1 include HBB, HBG1/2, ALAS2 and HMBS. Haploinsufficient heterozygous GATA1 knockout mice spontaneously develop erythroblastic leukemia, and GATA1-null mice die early in development due to profound anemia.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human GATA1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
For immunocytochemistry (ICC) use we recommend cell fixation/permeabilization with either PFA/Triton X-100 or PFA/methanol. Cell Fixation/permeabilization with methanol alone is not suitable for this antibody.
When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set.
Antigen Details
- Structure
- GATA1 is a 413 amino acid protein with a predicted molecular weight of 43 kD. Additional isoforms of 335 (35 kD) and 330 (34 kD) amino acids have been described.
- Distribution
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Erythroid and megakaryocytic cells/Nucleus
- Function
- Transcription Factor/Erythroid cell development and differentiation
- Cell Type
- Erythrocytes, Megakaryocytes
- Biology Area
- Cell Biology, Stem Cells, Transcription Factors
- Antigen References
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- Shimizu R, et al. 2008. Nat Rev Cancer. 8:279-87.
- Bresnick EH, et al. 2010. J Biol Chem. 285:31087-93.
- Katsumura, et al. 2017. Blood. 129:2092-2102.
- Gene ID
- 2623 View all products for this Gene ID
- UniProt
- View information about GATA1 on UniProt.org
Related FAQs
Other Formats
View All GATA1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GATA1 | P84F5 | WB,ICC,IP,ICFC |
PE anti-GATA1 | P84F5 | ICFC |
Alexa Fluor® 647 anti-GATA1 | P84F5 | ICFC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GATA1
Whole cell extracts (15 µg total protein) from K-562 and HEL... HeLa (negative control, panel A) and HEL (positive control, ... Whole cell extracts (250 µg total protein) prepared from HEL... HEL cells (filled histogram, positive control) or HeLa cells... -
PE anti-GATA1
HEL cells (positive control, filled histogram) and HeLa cell... -
Alexa Fluor® 647 anti-GATA1
HEL cells (positive control, filled histogram) and HeLa cell... HEL cells were fixed with Fixation Buffer (Cat. No. 420801) ...
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