- Clone
- FN50 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV A91
- Other Names
- Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_FN50_Alx647_070207 1_FN50_Alx647_070207](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/FN50_Alx647_070207.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 Alexa Fluor® 647 -
Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: HLA-DR (cyan) in Cycle 3, CD69 (red) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). -
Confocal image of human liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD163 (yellow) in Cycle 2 and CD49a (blue) in Cycle 4. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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310918 | 100 tests | $265 |
CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Additional Product Notes
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Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_528871 (BioLegend Cat. No. 310918)
Antigen Details
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
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Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479. - Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
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It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
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Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
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Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
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Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
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In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD69
PHA-activated human peripheral blood lymphocytes stained wit... -
FITC anti-human CD69
PMA + ionomycin stimulated (6 hours) human lymphocytes stain... -
PE anti-human CD69
PMA + ionomycin stimulated (6 hours) human lymphocytes stain... -
PE/Cyanine5 anti-human CD69
PHA-activated human peripheral blood lymphocytes stained wit... -
APC anti-human CD69
PMA+ionomycin-stimulated (5hours) human peripheral blood lym... -
APC/Cyanine7 anti-human CD69
PMA + Ionomycin stimulated (6 hours) human peripheral blood ... -
PE/Cyanine7 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes s... -
Alexa Fluor® 488 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes s... -
Alexa Fluor® 647 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes s... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... -
Pacific Blue™ anti-human CD69
PMA+ionomycin-stimulated human peripheral blood mononuclear ... -
Alexa Fluor® 700 anti-human CD69
PMA + Ionomycin-stimulated (5 hours) human peripheral blood ... -
Biotin anti-human CD69
PHA-stimulated human peripheral blood mononuclear cells (day... -
PerCP/Cyanine5.5 anti-human CD69
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
PerCP anti-human CD69
PMA + Inonomycin-stimulated (5 hours) human peripheral blood... -
Brilliant Violet 421™ anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA ... -
Brilliant Violet 785™ anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA ... -
Brilliant Violet 650™ anti-human CD69
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
Brilliant Violet 510™ anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA+... -
Brilliant Violet 605™ anti-human CD69
PMA+ ionomycin-stimulated (6 hours) human periphe... -
Purified anti-human CD69 (Maxpar® Ready)
Human PBMCs were incubated for 6 hours in media alone (botto... -
PE/Dazzle™ 594 anti-human CD69
PMA + ionomycin-stimulated (6 hours) human peripheral blood ... -
Brilliant Violet 711™ anti-human CD69
PMA + ionomycin-stimulated (six hours) human peripheral bloo... -
APC/Fire™ 750 anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA ... -
TotalSeq™-A0146 anti-human CD69
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TotalSeq™-B0146 anti-human CD69
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TotalSeq™-C0146 anti-human CD69
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Brilliant Violet 750™ anti-human CD69
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
KIRAVIA Blue 520™ anti-human CD69
PMA + ionomycin stimulated human peripheral blood lymphocyte... -
Spark NIR™ 685 anti-human CD69 Antibody
Human peripheral blood lymphocytes were stimulated with PMA+... -
PE/Fire™ 640 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes w... -
Spark YG™ 581 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes w... -
TotalSeq™-D0146 anti-human CD69
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APC anti-human CD69
Typical results from Cell Activation Cocktail (without Brefe... -
Spark Blue™ 550 anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA... -
PE anti-human CD69
Typical results from Cell Activation Cocktail (without Brefe... -
Spark Red™ 718 anti-human CD69
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
GMP PE anti-human CD69
Typical results from Cell Activation Cocktail (without Brefe... -
PE/Fire™ 810 anti-human CD69
PMA+ ionomycin stimulated (6 hours) human peripheral blood l... -
PE/Fire™ 744 anti-human CD69
Human peripheral blood lymphocytes were stained with anti-hu... PMA+ionomycin activated human peripheral blood lymphocytes w... -
Spark PLUS UV™ 395 anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA/...
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