- Clone
- 5F5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Spleen tyrosine kinase, protein tyrosine kinase SYK
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5F5_Purified_Syk_Antibody_1_032019 5F5_Purified_Syk_Antibody_1_032019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5F5_Purified_Syk_Antibody_1_032019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from A549, RPMI-8226, Daudi , and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with Purified anti-Syk Antibodies clones SYK-01 (0.5 µg/mL) and clone 5F5 (1.0 µg/mL), overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
Mouse spleen (1) and PBMC (2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with clone 5F5 (mouse-specific). Proteins were visualized using an HRP goat anti-mouse secondary antibody conjugate and a chemiluminescent system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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646001 | 25 µg | $124 | ||||
646002 | 100 µg | $265 |
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Syk peptide aa306-333
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
- Additional Product Notes
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In the first WB figure above, the cell lines selected for antibody testing were chosen to demonstrate specificity and species cross-reactivity, A549 was chosen as a human control cell line that does not express Syk protein; RPMI 8225 and Daudi are human cell lines that express Syk protein; and A20 cells is a mouse cell line that expresses Syk protein. Predicted SYK expression was obtained from Human Protein Atlas.
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Application References
(PubMed link indicates BioLegend citation) -
- Chu D, et al. 1999. J. Immunol. 163:2610.
- Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
- Product Citations
-
- RRID
-
AB_1659262 (BioLegend Cat. No. 646001)
AB_1659261 (BioLegend Cat. No. 646002)
Antigen Details
- Structure
- Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
- Distribution
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Cytoplasmic protein, widely expressed in hematopoietic cells
- Function
- Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
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1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe SL, et al. 2001.Immunology 103:164. - Gene ID
- 6850 View all products for this Gene ID
- UniProt
- View information about Syk on UniProt.org
Related FAQs
Other Formats
View All Syk Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Syk | 5F5 | WB,IP |
PE anti-Syk | 5F5 | ICFC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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