- Clone
- RM2-5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LFA-2, T11, Ly-37, SRBC-R
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RM2-5_PerCPCy5.5_CD2_Antibody_FC_042414 RM2-5_PerCPCy5.5_CD2_Antibody_FC_042414](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RM2-5_PerCPCy5.5_CD2_Antibody_FC_042414.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
100115 | 25 µg | $124 | ||||
100116 | 100 µg | $323 |
CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application Notes
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Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
- Ayroldi E, et al. 1997. Blood 89:3717. (Block)
- Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
- Cibotti R, et al. 1997. Immunity 6:245. (Costim)
- Product Citations
-
- RRID
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AB_2563501 (BioLegend Cat. No. 100115)
AB_2563502 (BioLegend Cat. No. 100116)
Antigen Details
- Structure
- Ig superfamily, 45-58 kD
- Distribution
-
T cells, thymocytes, NK cells, B cells
- Function
- T cell activation, adhesion
- Ligand/Receptor
- CD48, lck
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. - Gene ID
- 12481 View all products for this Gene ID
- UniProt
- View information about CD2 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD2 | RM2-5 | FC |
FITC anti-mouse CD2 | RM2-5 | FC |
PE anti-mouse CD2 | RM2-5 | FC |
Purified anti-mouse CD2 | RM2-5 | FC,IP,Block,Costim |
APC anti-mouse CD2 | RM2-5 | FC |
PE/Cyanine7 anti-mouse CD2 | RM2-5 | FC |
PerCP/Cyanine5.5 anti-mouse CD2 | RM2-5 | FC |
TotalSeq™-A0892 anti-mouse CD2 | RM2-5 | PG |
Ultra-LEAF™ Purified anti-mouse CD2 | RM2-5 | FC,IP,Block,Costim |
TotalSeq™-C0892 anti-mouse CD2 | RM2-5 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD2
C57BL/6 mouse splenocytes stained with biotinylated RM2-5, f... -
FITC anti-mouse CD2
C57BL/6 mouse splenocytes stained with RM2-5 FITC -
PE anti-mouse CD2
C57BL/6 mouse splenocytes stained with RM2-5 PE -
Purified anti-mouse CD2
C57BL/6 mouse splenocytes stained with purified RM2-5 -
APC anti-mouse CD2
C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-... -
PE/Cyanine7 anti-mouse CD2
C57BL/B6 mouse splenocytes were stained with CD2 (clone RM2-... -
PerCP/Cyanine5.5 anti-mouse CD2
C57BL/6 mouse splenocytes were stained with CD2 (clone RM2-5... -
TotalSeq™-A0892 anti-mouse CD2
-
Ultra-LEAF™ Purified anti-mouse CD2
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
TotalSeq™-C0892 anti-mouse CD2
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