- Clone
- B3B4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![B3B4_BV421_CD23_Antibody_FC_051414 B3B4_BV421_CD23_Antibody_FC_051414](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/B3B4_BV421_CD23_Antibody_FC_051414.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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101621 | 50 µg | $216 |
CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, immunohistochemical staining of acetone-fixed frozen sections5, and spatial biology (IBEX)7,8.
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Application References
(PubMed link indicates BioLegend citation) -
- Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
- Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
- Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
- Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
- Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
- Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
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AB_2563599 (BioLegend Cat. No. 101621)
Antigen Details
- Structure
- C-lectin family, type II transmembrane glycoprotein, 45 kD
- Distribution
-
B cells, follicular dendritic cells
- Function
- Regulates B cell activation
- Ligand/Receptor
- IgE, CD19/CD21/CD81
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038. - Gene ID
- 14128 View all products for this Gene ID
- UniProt
- View information about CD23 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD23 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD23 | B3B4 | FC |
FITC anti-mouse CD23 | B3B4 | FC |
PE anti-mouse CD23 | B3B4 | FC |
Purified anti-mouse CD23 | B3B4 | FC,IP,Block,IHC-F |
Alexa Fluor® 488 anti-mouse CD23 | B3B4 | FC |
Alexa Fluor® 647 anti-mouse CD23 | B3B4 | FC,IHC-F,SB |
PE/Cyanine7 anti-mouse CD23 | B3B4 | FC |
Pacific Blue™ anti-mouse CD23 | B3B4 | FC |
PerCP/Cyanine5.5 anti-mouse CD23 | B3B4 | FC |
APC anti-mouse CD23 | B3B4 | FC |
Brilliant Violet 421™ anti-mouse CD23 | B3B4 | FC |
Brilliant Violet 510™ anti-mouse CD23 | B3B4 | FC |
Purified anti-mouse CD23 (Maxpar® Ready) | B3B4 | FC,CyTOF® |
APC/Cyanine7 anti-mouse CD23 | B3B4 | FC |
Alexa Fluor® 700 anti-mouse CD23 | B3B4 | FC |
PE/Dazzle™ 594 anti-mouse CD23 | B3B4 | FC |
TotalSeq™-A0108 anti-mouse CD23 | B3B4 | PG |
Brilliant Violet 605™ anti-mouse CD23 | B3B4 | FC |
TotalSeq™-C0108 anti-mouse CD23 | B3B4 | PG |
APC/Fire™ 750 anti-mouse CD23 Antibody | B3B4 | FC |
TotalSeq™-B0108 anti-mouse CD23 | B3B4 | PG |
Brilliant Violet 785™ anti-mouse CD23 | B3B4 | FC |
Brilliant Violet 711™ anti-mouse CD23 | B3B4 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD23
C57BL/6 mouse splenocytes were stained with biotinylated CD2... -
FITC anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... -
PE anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... -
Purified anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with purified CD23 (... -
Alexa Fluor® 488 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... -
Alexa Fluor® 647 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Mice were injected subcutaneously with sheep red blood cells... -
PE/Cyanine7 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... -
Pacific Blue™ anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B... -
PerCP/Cyanine5.5 anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
APC anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 421™ anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4... -
Brilliant Violet 510™ anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4... -
Purified anti-mouse CD23 (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
APC/Cyanine7 anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4... -
Alexa Fluor® 700 anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4... -
PE/Dazzle™ 594 anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4... -
TotalSeq™-A0108 anti-mouse CD23
-
Brilliant Violet 605™ anti-mouse CD23
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
TotalSeq™-C0108 anti-mouse CD23
-
APC/Fire™ 750 anti-mouse CD23 Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
TotalSeq™-B0108 anti-mouse CD23
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Brilliant Violet 785™ anti-mouse CD23
C57BL/6 splenocytes were stained with anti-mouse/human CD45R... -
Brilliant Violet 711™ anti-mouse CD23
C57BL/6 mouse splenocytes were stained with anti-mouse/human...
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