BioLegend Biomarkers and Immunoassays
Our family of products under Biomarkers and Immunoassays includes reagents for the study of soluble proteins, typically accessible in blood, urine, saliva, or other secretions, as well as those found in tissues or inside cells. These soluble proteins, whether they are chemokines, cytokines, growth factors, or other secreted proteins play many important roles in the development, function, and immunity of the organism, as well as serving as biomarkers in disease states. Here, we offer an extensive variety of reagents for the quantitation and study of these proteins, including multiplex bead-based immunoassays, purified and conjugated antibodies, ELISA kits and sets, recombinant protein standards, and much more.
Multiplex Kits   Antibodies   ELISA Kits and Sets   Recombinant Protein Standards   Product Citations
BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. The assay utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.

LEGENDplex™ kits are provided with predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system. Custom options are also available.

Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate or v-bottom plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.

Software is downloaded for free and each kit comes with a dongle key required for use.

Learn more about LEGENDplex™
LEGENDplex™ Predefined Panels

The LEGENDplex™ panels cover a wide range of soluble markers for immunology, growth factors, and metabolism. Currently, reactivities include human, mouse, rat, and non-human primate species (Rhesus and Cynomolgus). Panels are carefully selected for important biomarkers that are often analyzed together in a set.
HumanMouseMouse/RatNHPRat
T Helper Cytokine Panels
Proinflammatory Chemokine Panel
Cytokine Panel 2
Inflammation Panel
Growth Factor Panel
Adipokine Panel
Metabolic Panel 1
CD8/NK Panel
Anti-Virus Response Panel
Apolipoprotein (Apo) Panel
Human/NHP Free Active/Total TGF-β1 Panel
TIMP Panel
IGFBP Panel
B Cell Panels
Macrophage/Microglia Panels
T Helper Cytokine Panels
Proinflammatory Chemokine Panel
Cytokine Panel 2
Inflammation Panel
Immunoglobulin Isotyping Panel
Free Active/Total TGF-β1 Panel
T Helper Cytokine Panels
Chemokine/Cytokine Panel
Inflammation Panel
Growth Factor Panel
Adipokine Panel
T Helper Cytokine Panels
Inflammation Panel
View panel details…
LEGENDplex™ Mix and Match

Our Mix and Match system is more suited for researchers requiring flexibility in the in their choice of analytes. Instead of ordering all 13 specificities in a predefined panel, you can choose any subset within a panel, even a single analyte if desired. The system is easy to use and cost-effective. Just order the analyte capture beads, the appropriate lyophilized standard, the appropriate detection antibodies, and the appropriate buffer set.

Mix and Match kits must be ordered using the LEGENDplex™ page.

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