- Clone
- TC15-12F12.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TC15-12F12dot2_LEAF_957 TC15-12F12dot2_LEAF_957](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TC15-12F12dot2_LEAF_957.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
115949 | 100 µg | 199€ | ||||
115950 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse SLAM-human IgG1 fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IP, Costim, Block - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).
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Application References
(PubMed link indicates BioLegend citation) -
- Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
- Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
- Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
- Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
- Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
- Jung Y, et al. 2007. Blood 110:82. PubMed
- Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
- Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
- Kim I, et al. 2006. Blood 108:737. PubMed
- Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
- Fraser ST, et al. 2007. Blood 109:4616. PubMed
- Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
- Song J, et al. 2010. Blood 115:2592. PubMed
- Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
- Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
- Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
- RRID
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AB_2832395 (BioLegend Cat. No. 115949)
AB_2832396 (BioLegend Cat. No. 115950)
Antigen Details
- Structure
- Ig superfamily, 75-95 kD
- Distribution
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Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- Function
- B cell and dendritic cell costimulation
- Ligand/Receptor
- CD150
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614. - Gene ID
- 27218 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD150 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with purified CD150 (... -
PE anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Biotin anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with biotinylated CD1... -
APC anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
PE/Cyanine5 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
PE/Cyanine7 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Alexa Fluor® 488 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Alexa Fluor® 647 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
PerCP/Cyanine5.5 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Pacific Blue™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... -
Brilliant Violet 421™ anti-mouse CD150 (SLAM)
C57BL/6 mouse bone marrow cells were stained with SLAM (clon... C57BL/6 mouse splenocytes were stained with SLAM (clone TC15... -
Brilliant Violet 605™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... -
Brilliant Violet 510™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with SLAM (clone TC15... -
Brilliant Violet 650™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Purified anti-mouse CD150 (SLAM) (Maxpar® Ready)
Mouse bone marrow cells stained with 156Gd anti-CD48 (HM48.1... -
PE/Dazzle™ 594 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
Brilliant Violet 785™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
APC/Fire™ 750 anti-mouse CD150 (SLAM)
C57BL/6 Mouse splenocytes were stained with CD150 (SLAM) (cl... C57BL/6 mouse bone marrow cells were stained with CD150 (SLA... -
Brilliant Violet 711™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC1... C57BL/6 mouse bone marrow cells were stained with CD150 (clo... -
TotalSeq™-A0203 anti-mouse CD150 (SLAM)
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TotalSeq™-C0203 anti-mouse CD150 (SLAM)
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Ultra-LEAF™ Purified anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ puri... -
TotalSeq™-B0203 anti-mouse CD150 (SLAM)
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