- Clone
- C9B7W (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LAG3, LAG-3
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![C9B7W_PURE_CD223_Antibody_1_091719 C9B7W_PURE_CD223_Antibody_1_091719](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/C9B7W_PURE_CD223_Antibody_1_091719.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD3ε (clone 145-2C11) APC and CD223 (LAG-3) (clone C9B7W) Purified (left) or rat IgG1, κ Purified isotype control (right) followed by anti-Rat IgG PE.
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CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine CD223-Ig fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- RRID
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AB_2814003 (BioLegend Cat. No. 125235)
AB_2814004 (BioLegend Cat. No. 125236)
AB_2814002 (BioLegend Cat. No. 125234)
AB_2813999 (BioLegend Cat. No. 125231)
AB_2814000 (BioLegend Cat. No. 125232)
AB_2814001 (BioLegend Cat. No. 125233)
Antigen Details
- Structure
- Ig superfamily
- Distribution
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T cells and NK cells, as well as T regulatory cells
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
- Gene ID
- 16768 View all products for this Gene ID
- UniProt
- View information about CD223 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD223 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained ... -
Biotin anti-mouse CD223 (LAG-3)
ConA-stimulated (3days) C57BL/6 splenocytes were stained wit... -
PE anti-mouse CD223 (LAG-3)
ConA-stimulated C57BL/6 splenocytes stained with C9B7W PE -
APC anti-mouse CD223 (LAG-3)
Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9... -
PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3)
Con A-stimulated splenocytes stained with anti-mouse CD223 P... -
Brilliant Violet 785™ anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
GoInVivo™ Purified anti-mouse CD223 (LAG-3)
Anti-mouse LAG-3, or CD223, inhibits the binding of mouse LA... -
Brilliant Violet 421™ anti-mouse CD223 (LAG-3)
Con A + IL-2-stimulated C57BL/6 splenocytes (3 days) were st... -
PE/Dazzle™ 594 anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
Brilliant Violet 650™ anti-mouse CD223 (LAG-3)
Con A + IL-2-stimulated C57BL/6 splenocytes (3 days) were st... -
PE/Cyanine7 anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
TotalSeq™-A0378 anti-mouse CD223 (LAG-3)
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Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained ... -
TotalSeq™-C0378 anti-mouse CD223 (LAG-3)
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APC/Fire™ 750 anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
Alexa Fluor® 647 anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
TotalSeq™-B0378 anti-mouse CD223 (LAG-3)
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Brilliant Violet 711™ anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
PE/Fire™ 640 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
PerCP/Fire™ 806 anti-mouse CD223 (LAG-3)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
PerCP/Fire™ 780 anti-mouse CD223 (LAG-3)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
Spark Red™ 718 anti-mouse CD223 (LAG-3) (Flexi-Fluor™)
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PE/Cyanine5 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
PE/Fire™ 810 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
Brilliant Violet 605™ anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated (3 days) C57BL/6 splenocytes were stai...
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