- Clone
- EH12.2H7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD-1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Anti-human PD-1 inhibits the binding of PD-L1. Immobilized PD-1-Fc was pre-incubated with increasing concentrations of anti-human PD-1 (clone EH12.2H7, purple squares) or isotype control (clone MOPC-21, purple circles), followed by incubation with a fixed concentration of PD-L1-Fc (1 µg/mL). Clone EH12.2H7 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
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Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Block, IHC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
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GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3 and immunohistochemical staining of paraformaldehyde fixed frozen sections13. -
Application References
(PubMed link indicates BioLegend citation) -
- Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
- Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
- Velu V, et al. 2007. J. Virol. 81:5819. (FA)
- Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
- Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
- Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
- Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
- Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
- Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
- Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
- Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
- Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Product Citations
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- RRID
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AB_2565904 (BioLegend Cat. No. 329943)
AB_2566079 (BioLegend Cat. No. 329945)
AB_2566078 (BioLegend Cat. No. 329944)
AB_2566292 (BioLegend Cat. No. 329948)
AB_2566291 (BioLegend Cat. No. 329947)
AB_2566290 (BioLegend Cat. No. 329946)
Antigen Details
- Structure
- Immunoglobulin superfamily
- Distribution
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Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
- Ligand/Receptor
- B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Gene ID
- 5133 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
Other Formats
View All CD279 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 421™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Purified anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... -
FITC anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD279 (... -
PE anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD279 (... Confocal image of human lymph node sample acquired using the... -
APC anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD279 (... -
Alexa Fluor® 647 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD279 (... -
PerCP/Cyanine5.5 anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Cyanine7 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes st... -
Pacific Blue™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD279 (... PHA-stimulated (day-3) human peripheral blood lymphocytes we... -
PE/Cyanine7 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with CD279 (... -
Purified anti-human CD279 (PD-1) (Maxpar® Ready)
Human PBMCs were incubated for 3 days in media alone (top) o... -
Brilliant Violet 605™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Ultra-LEAF™ Purified anti-human CD279 (PD-1)
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Brilliant Violet 711™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 785™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 510™ anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes we... -
Biotin anti-human CD279 (PD-1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Dazzle™ 594 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 APC... PHA-stimulated (day 3) human peripheral blood lymphocytes st... -
Alexa Fluor® 488 anti-human CD279 (PD-1)
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
PerCP anti-human CD279 (PD-1)
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
GoInVivo™ Purified anti-human CD279 (PD-1)
Anti-human PD-1 inhibits the binding of PD-L1. Immobilized P... -
Brilliant Violet 650™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (three days) human peripheral blood lymphocyt... -
Alexa Fluor® 700 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (three days) human peripheral blood lymphocyt... -
APC/Fire™ 750 anti-human CD279 (PD-1)
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
TotalSeq™-A0088 anti-human CD279 (PD-1)
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TotalSeq™-B0088 anti-human CD279 (PD-1)
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TotalSeq™-C0088 anti-human CD279 (PD-1)
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Brilliant Violet 750™ anti-human CD279 (PD-1)
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
TotalSeq™-D0088 anti-human CD279 (PD-1)
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PE/Fire™ 640 anti-human CD279 (PD-1)
PHA stimulated (day 3) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine5 anti-human CD279 (PD-1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Fire™ 744 anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD279 (PD-1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w...
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