Purified anti-human/mouse SSEA-3 Antibody anti-SSEA-3

Pricing & Availability

Clone: MC-631 (See other available formats)
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen-3
Isotype: Rat IgM, κ
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Product Citations: publications

NCCIT, human teratocarcinoma cell line stained with purified MC-631, followed by anti-rat Ig FITC

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330302

100 µg

216€

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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Mouse

Reported Reactivity

African Green

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

4-8 cell stage mouse embryos

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
ICC, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy¹.

Application References (PubMed link indicates BioLegend citation)

Kannagi R, et al. 1983. EMBO 2:2355. (IF)
Kaufmann B, et al. 2005. Virology 332:189. (ELISA)

Product Citations

Oguma Y, et al. 2022. iScience. 25:105395. PubMed
Hey CAB et al. 2018. Stem Cell Res. 33:46-50 . PubMed
Hey CAB et al. 2018. Stem cell research. 31:235-239 . PubMed
Hori Y, et al. 2022. Front Cardiovasc Med. 9:981088. PubMed
Fujita Y, et al. 2021. J Invest Dermatol. 198:141. PubMed
Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
Schmid B et al. 2019. Stem Cell Res. 1723:09:00 . PubMed

RRID

AB_1236554 (BioLegend Cat. No. 330302)

Antigen Details

Distribution: Embryonic stemcells
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells
Gene ID: 93961 View all products for this Gene ID
UniProt: View information about SSEA-3 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All SSEA-3 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human/mouse SSEA-3	MC-631	FC
Alexa Fluor® 488 anti-human/mouse SSEA-3	MC-631	FC
Purified anti-human/mouse SSEA-3	MC-631	FC,ICC,ELISA
Alexa Fluor® 647 anti-human/mouse SSEA-3	MC-631	FC
APC/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC
Biotin anti-human/mouse SSEA-3	MC-631	FC
FITC anti-human/mouse SSEA-3	MC-631	FC
PerCP/Cyanine5.5 anti-human/mouse SSEA-3	MC-631	FC
PE/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 05.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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