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Chloroquine Phosphate

Pricing & Availability
Regulatory Status
RUO
Other Names
Chloroquine, CQ
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Product Citations
publications
Chloroquine_Phosphate_1_WB_050720
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 50 µM chloroquine phosphate for 18 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a purified anti-p62 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • Chloroquine_Phosphate_1_WB_050720
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 50 µM chloroquine phosphate for 18 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a purified anti-p62 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • Chloroquine_Phosphate_2_ICC_050720
    HeLa cells were grown in media with 50 µM (panel B) or without (panel A) chloroquine phosphate for 18 hour. Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a purified anti-p62 antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 1:200 dilution. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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427502 100 mg £61
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Description

Chloroquine phosphate is used as an anti-malarial drug. It inhibits proinflammatory cytokine levels and is being used as a treatment to decrease inflammation in autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. The drug is also broadly used to study autophagic pathways in eukaryotic cells, where it blocks lysosomal acidification. This decreases autophagic flux by disrupting autophagasome-lysosome fusion. Additionally, chloroquine phosphate inhibits infection by SARS-CoV-2 and other coronaviruses in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Lyophilized
Preparation
Resuspend 100 mg of lyophilized chloroquine phosphate in 3.87 mL of ddH2O to make 50 mM stock concentration.
Storage & Handling
Store lyophilized powder at room temperature under desiccation. Protect from light. Once reconstituted, store at -20°C. Use reconstituted material within 3 months to avoid loss of potency.
Application

Activation - Verified
Recommended Usage

Treatment of cultured cells with chloroquine phosphate ranges from 10-50 μM for 12-48 hours. It is recommended that the reagent be titrated to determine optimal performance.

Disclaimer

This product is for research use. It is not intended for therapeutic use or human consumption.

Antigen Details

Biology Area
Cell Biology, COVID-19
Antigen References
  1. Jiang PD, et al. 2010. Biomed. Pharmacother. 64:609.
  2. Frieboes HB, et al. 2014. JOP. 15:189.
  3. Levy JM, et al. 2014. Cancer Discov. 4:773.
  4. Wang M, et al. 2020. Cell Res. 30:269.
Gene ID
NA

Related FAQs

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Go To Top Version: 2    Revision Date: 08/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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