- Clone
- A18246G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_A18246G_ULEAF_CD25_Antibody_1_061323 A_A18246G_ULEAF_CD25_Antibody_1_061323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18246G_ULEAF_CD25_Antibody_1_061323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clone A18246G) (left) or 250 µg Ultra-LEAF™ Purified rat IgG2b, κ isotype control (clone RTK4530) (right) were intraperitoneally (IP) injected to C57BL/6 mice, and splenocytes analyzed on day 7 post-injection. Cells shown are gated CD3+ splenocyte population. -
% CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneally injected with 250 µg Ultra-LEAF™ Rat IgG2b, κ Isotype Ctrl (clone RTK4530) (black line) or Ultra-LEAF™ anti-mouse CD25 (clone A18246G) (purple line) at indicated days post-injection. Error bars represent standard deviation from 3 mice analyzed per condition and timepoint.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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112603 | 100 µg | £143 | ||||
112604 | 1 mg | £411 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD25, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac, is a 55 kD glycoprotein. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
Depletion - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For in vivo depletion, suggested use is 250 µg of Ultra-LEAF™ purified of clone A18246G for one C57BL/6 mouse intraperitoneal injection. It is recommended that the reagent be titrated for optimal performance.
- Additional Product Notes
-
Ultra-LEAF™ purified (clone A18246G) antibody recognizes a different epitope compared to other mouse CD25 such as clones 3C7 and PC61.
Ultra-LEAF™ purified (clone A18246G) antibody can deplete CD25+ cells in vivo in C57BL/6 mouse by intraperitoneal injection. - RRID
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AB_2941391 (BioLegend Cat. No. 112603)
AB_2941391 (BioLegend Cat. No. 112604)
Antigen Details
- Structure
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
- Distribution
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Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- Function
- IL-2 receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
- Taniguchi T, et al. 1993. Cell. 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD25 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-mouse CD25 | A18246G | FC,IP |
Spark PLUS UV™ 395 anti-mouse CD25 | A18246G | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD25
250 µg of Ultra-LEAF™ Purified anti-mouse CD25 antibody (clo... % CD3+CD4+CD25+ splenocytes analyzed in mice intraperitoneal... -
Spark PLUS UV™ 395 anti-mouse CD25
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 (...
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