- Clone
- D665.Rec (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tp44, T44
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![D665Rec_Ultra-LEAF_-CD28_Antibody_053123 D665Rec_Ultra-LEAF_-CD28_Antibody_053123](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/D665Rec_Ultra-LEAF_-CD28_Antibody_053123-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ Purified anti-mouse CD28 (clone D665.Rec) (left) or purified mouse IgG1, κ isotype control (right) followed by PE anti-mouse IgG1. Cells were costained with anti-mouse CD3 APC. -
Total T-cells from C57BL/6 mouse splenocytes were cultured with Ultra-LEAF™ purified anti-mouse CD28 (clone D665.Rec) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram) for 3 days. Cells were fixed and permeabilized with 70% ethanol, and then stained intracellularly with anti-mouse Ki-67 Alexa Fluor® 647. Data shown are CD4+ cells. -
Total T-cells from C57BL/6 mouse splenocytes were labeled with CFSE, and stimulated with Ultra-LEAF™ purified anti-mouse CD28 (clone D665.Rec) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram) for 4 days. Data shown are CD4+ cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
117003 | 100 µg | £76 | ||||
117004 | 1 mg | £102 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD28, also known as Tp44 or T44, is a 44 kD glycoprotein. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The clone D665.Rec is able to stimulate mouse T cells to proliferate without TCR or CD3 stimulation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone D665.Rec can completely block the binding of clone D665 on target cells.
Clone D665.Rec can induce proliferation of mouse T cells without TCR or anti-CD3 stimulation. - RRID
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AB_3068072 (BioLegend Cat. No. 117003)
AB_3068072 (BioLegend Cat. No. 117004)
Antigen Details
- Structure
- Ig superfamily, 44 kD
- Distribution
-
Thymocytes, peripheral T cells
- Function
- Costimulates T
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Adaptive Immunity, Costimulatory Molecules, Immuno-Oncology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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- Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Lenschow DJ, et al. 1996. Annu Rev Immunol. 14:233-58.
- Gross JA, et al. 1992. J Immunol. 149:380-8.
- Gene ID
- 12487 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD28 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-mouse CD28 (Superagonistic) Recombinant Antibody | D665.Rec | FC,Activ |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD28 (Superagonistic) Recombinant Antibody
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ Puri... Total T-cells from C57BL/6 mouse splenocytes were cultured w... Total T-cells from C57BL/6 mouse splenocytes were labeled wi...
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