- Clone
- HIP8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV P38
- Other Names
- gpIIb, CD41a
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIP8_LEAF_021508.jpg HIP8_LEAF_021508.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIP8_LEAF_021508.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood platelets stained with Ultra-LEAF™ Purified HIP8, followed by anti-mouse IgG FITC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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303745 | 100 µg | £185 | ||||
303746 | 1 mg | £509 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD41 is a 125/25 kD α subunit of the gpIIb/IIIa (CD41/CD61) complex. CD41 is a heterodimer composed of a heavy chain (gpIIbα) and light chain (gpIIbβ) linked by a single disulfide bond. It is a member of the integrin family primarily expressed on platelets and megakaryocytes. CD41 has been reported to be involved with platelet aggregation and platelet attachment to the ECM. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, and thrombin.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
CyTOF® - Verified
Block, IHC-F - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and blocking of platelet aggregation2. The HIP8 antibody has been reported to block the activation of platelets by various stimuli, including collagen, and ADP.
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- McCarty OJT, et al. 2000. Blood 96:1789.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zhi L et al. 2013. PLoS One. 8:e79869. (IHC)
- RRID
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AB_2832588 (BioLegend Cat. No. 303745)
AB_2832589 (BioLegend Cat. No. 303746)
Antigen Details
- Structure
- Integrin family, α subunit of CD41/CD61 (GPIIb-IIIa) complex, 125/22 kD
- Distribution
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Platelets, megakaryocytes
- Function
- Platelet aggregation, platelet attachment to extracellular matrix
- Ligand/Receptor
- Fibrinogen, fibronectin, von Willebrand factor, thrombin
- Cell Type
- Megakaryocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Denzin L, et al. 1996. J. Exp. Med. 184:2153.
2. Denzin L, et al. 1995. Cell 82:155.
3. Riberdy J, et al. 1994. J. Cell Biol. 125:1225. - Gene ID
- 3674 View all products for this Gene ID
- UniProt
- View information about CD41 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD41 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD41
Human platelets stained with HIP8 PE -
FITC anti-human CD41
Human peripheral blood platelets stained with HIP8 FITC -
PE anti-human CD41
Human platelets stained with HIP8 PE -
PE/Cyanine5 anti-human CD41
Human peripheral blood platelets stained with HIP8 PE/Cyanin... -
Purified anti-human CD41
Human peripheral blood platelets stained with HIP8, followed... -
Pacific Blue™ anti-human CD41
Human peripheral blood platelets were stained with CD41 (cl... -
APC/Cyanine7 anti-human CD41
Human platelets were stained with CD41 (clone HIP8) APC/Cyan... -
PE/Cyanine7 anti-human CD41
Human platelets were stained with CD41 (clone HIP8) APC/Cyan... -
PerCP/Cyanine5.5 anti-human CD41
Human peripheral blood platelets were stained with CD41 (clo... -
Purified anti-human CD41 (Maxpar® Ready)
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Alexa Fluor® 647 anti-human CD41
Human platelets were stained with CD41 (clone HIP8) Alexa Fl... -
Alexa Fluor® 700 anti-human CD41
Human platelets were stained with CD41 (clone HIP8) Alexa Fl... -
Alexa Fluor® 488 anti-human CD41
Human platelets were stained with CD41 (clone HIP8) Alexa Fl... -
Brilliant Violet 421™ anti-human CD41
Human peripheral blood platelets were stained with CD41 (clo... -
PE/Dazzle™ 594 anti-human CD41
Human peripheral blood platelets were stained with anti-huma... -
Brilliant Violet 510™ anti-human CD41
Human platelets were stained with CD41 (clone HIP8) Brillian... -
Biotin anti-human CD41
Human peripheral blood platelets were stained with biotinyla... -
TotalSeq™-A0353 anti-human CD41
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TotalSeq™-C0353 anti-human CD41
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Brilliant Violet 785™ anti-human CD41
Human peripheral blood platelets were stained with anti-huma... -
Brilliant Violet 605™ anti-human CD41
Human peripheral blood platelets were stained with anti-huma... -
Ultra-LEAF™ Purified anti-human CD41
Human peripheral blood platelets stained with Ultra-LEAF™ Pu... -
TotalSeq™-B0353 anti-human CD41 Antibody
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APC/Fire™ 750 anti-human CD41 Antibody
Human platelets were stained with anti-human CD41 (clone HIP... -
TotalSeq™-D0353 anti-human CD41
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APC anti-human CD41
Typical results from Human peripheral blood platelets staine... -
FITC anti-human CD41
Typical results from Human peripheral blood platelets staine... -
GMP APC anti-human CD41
Typical results from Human peripheral blood platelets staine...
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