- Clone
- 9F.8A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7-H2, B7H2, B7-RP1, ICOSL, ICOS-L
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_9F.8A4_ULEAF_CD275_Antibody_1_FC_061417 A_9F.8A4_ULEAF_CD275_Antibody_1_FC_061417](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9Fdot8A4_ULEAF_CD275_Antibody_1_FC_061417.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood leukocytes were stained with Ultra-LEAF™ Purified CD275 (clone 9F.8A4) (top) or Ultra-LEAF™ Purified mouse IgG1, κ isotype control (bottom), followed by PE goat anti-mouse IgG. The cells were then stained with CD19 FITC. Data shown were gated on lymphocyte population. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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329808 | 1 mg | £433 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human B7H2-mIg fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Qian X, et al. 2006. Eur J Immunol. 36:906-918.
- Ribot JC, et al. 2012. J. Immunol. 189:1202. PubMed.
- Product Citations
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- RRID
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AB_2687113 (BioLegend Cat. No. 329808)
Antigen Details
- Structure
- Ig superfamily, B7 family, 40 kD
- Distribution
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B cells and dendritic cells
- Function
- Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
- Ligand/Receptor
- ICOS
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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Qian X, et al. 2006. Eur J Immunol. 36:906-918.
- Gene ID
- 23308 View all products for this Gene ID
- UniProt
- View information about CD275 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD275 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD275 (B7-H2, B7-RP1, ICOSL) | 9F.8A4 | FC |
PE anti-human CD275 (B7-H2, B7-RP1, ICOSL) | 9F.8A4 | FC |
Ultra-LEAF™ Purified anti-human CD275 (B7-H2, B7-RP1, ICOSL) | 9F.8A4 | FC |
TotalSeq™-A0172 anti-human CD275 (B7-H2, B7-RP1, ICOSL) | 9F.8A4 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD275 (B7-H2, B7-RP1, ICOSL)
Human peripheral blood lymphocytes stained with purified 9F.... -
PE anti-human CD275 (B7-H2, B7-RP1, ICOSL)
Human peripheral blood lymphocytes stained with 9F.8A4 PE an... -
Ultra-LEAF™ Purified anti-human CD275 (B7-H2, B7-RP1, ICOSL)
Human peripheral blood leukocytes were stained with Ultra-LE... -
TotalSeq™-A0172 anti-human CD275 (B7-H2, B7-RP1, ICOSL)
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