- Clone
- MHN1-519 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Neurogenic locus notch homolog protein 1 (Notch 1), Translocation-associated notch protein (TAN-1), Motch A, mT14, p300
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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352111 | 100 µg | £185 | ||||
352112 | 1 mg | £509 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Notch1-Fc fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking Notch 1 mediated binding to DLL4 in human cord blood CD34+ cells1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Haraguchi K, et al. 2009. J. Immunol. 182:6168. (Block)
- Yamanda S, et al. 2009. Blood 113:3631. (FC)
- Guy CS. et al. 2013. Nat Immunol. 14:262. PubMed
- RRID
-
AB_2860916 (BioLegend Cat. No. 352111)
AB_2860917 (BioLegend Cat. No. 352112)
Antigen Details
- Structure
- Transmembrane protein. The extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 CDC10/ANK, 1 PEST motif, and 1 RAM23 domain.
- Distribution
-
Highly expressed in the brain, lung, and thymus. Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
- Function
- Regulates development, differentiation, and survival of a broad spectrum of cell lineages. Involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
- Interaction
-
RBP-J κ.
- Ligand/Receptor
- Jagged 1, Jagged 2, Delta 1, Delta 4.
- Cell Type
- B cells, Neural Stem Cells, Thymocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Antigen References
-
1. Vicente R, et al. 2010. Semin. Immunol. 22:270.
2. Zhao WL. 2010. Leukemia 24:13.
3. Sanda T, et al. 2010. Blood 115:1735.
4. Zhou J, et al. 2009. Immunity 31:356. - Gene ID
- 4851 View all products for this Gene ID
- UniProt
- View information about Notch 1 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Notch 1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Notch 1 | MHN1-519 | FC,Block |
PE anti-human Notch 1 | MHN1-519 | FC |
APC anti-human Notch 1 | MHN1-519 | FC |
TotalSeq™-A0213 anti-human Notch 1 | MHN1-519 | PG |
Ultra-LEAF™ Purified anti-human Notch 1 | MHN1-519 | FC,Block |
TotalSeq™-C0213 anti-human Notch 1 | MHN1-519 | PG |
TotalSeq™-B0213 anti-human Notch 1 | MHN1-519 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Notch 1
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human Notch 1 transfected CHO cells were stained with Notch ... -
PE anti-human Notch 1
PHA-stimulated (3 days) human peripheral blood lymphocytes w... Human Notch 1 transfected CHO cells were stained with Notch ... -
APC anti-human Notch 1
Human Notch 1 transfected CHO cells were stained with Notch ... -
TotalSeq™-A0213 anti-human Notch 1
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Ultra-LEAF™ Purified anti-human Notch 1
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TotalSeq™-C0213 anti-human Notch 1
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TotalSeq™-B0213 anti-human Notch 1
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