- Clone
- S18024F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Toll-like receptor (TLR) 7, CD287
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S18024F_ULEAF_TLR7_Antibody_042022 S18024F_ULEAF_TLR7_Antibody_042022](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S18024F_ULEAF_TLR7_Antibody_042022-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human 293XL-hTLR7 transfected cells were treated with R848 only (red Bar) or R848 plus 1 µg of Ultra-LEAF™ purified rat IgG2a, κ isotype control (yellow bar) or R848 plus 10, 1, 0.1 or 0.01 µg of Ultra-LEAF™ purified anti-human TLR7 (clone S18024F) (purple bars). Supernatant was harvested next day and analyzed using the ELISA MAX™ Deluxe Set Human IL-8 (Cat. No. 431504). -
Human peripheral blood plasmacytoid dendritic cells were stained with anti-human CD304 (clone 12C2), fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set, and intracellularly stained with anti-human TLR7 (clone S18024F) Ultra-LEAF™ (closed histogram), or purified rat IgG2a, κ isotype control (open histogram) followed by PE anti-rat IgG2a.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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376911 | 100 µg | £185 | ||||
376912 | 1 mg | £509 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Block - Quality tested
ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by blocking. > 80% blocking was observed with 1 µg antibody per well. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ug per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.
- RRID
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AB_2936679 (BioLegend Cat. No. 376911)
AB_2936679 (BioLegend Cat. No. 376912)
Antigen Details
- Structure
- Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
- Distribution
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Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes
- Function
- Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
- Interaction
- B cells, Dendritic cells
- Ligand/Receptor
- Single-stranded RNA
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Innate Immune Signaling
- Gene ID
- 51284 View all products for this Gene ID
- UniProt
- View information about TLR7 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All TLR7 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human TLR7 | S18024F | ICFC |
FITC anti-human TLR7 | S18024F | ICFC |
Brilliant Violet 421™ anti-human TLR7 | S18024F | ICFC |
Ultra-LEAF™ Purified anti-human TLR7 | S18024F | Block,ICFC |
APC anti-human TLR7 | S18024F | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human TLR7
Human peripheral blood plasmacytoid dendritic cells were sta... -
FITC anti-human TLR7
Human peripheral blood plasmacytoid dendritic cells were sta... -
Brilliant Violet 421™ anti-human TLR7
Human peripheral blood plasmacytoid dendritic cells were sta... -
Ultra-LEAF™ Purified anti-human TLR7
Human 293XL-hTLR7 transfected cells were treated with R848 o... Human peripheral blood plasmacytoid dendritic cells were sta... -
APC anti-human TLR7
Human peripheral blood plasmacytoid dendritic cells were sta...
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