- Clone
- QA19A67 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA19A67_ULEAF_GM-CSF_Antibody040622 QA19A67_ULEAF_GM-CSF_Antibody040622](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA19A67_ULEAF_GM-CSF_Antibody040622-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant mouse GM-CSF (Cat. No. 576306) (black circles) induces the proliferation of FDC-P1 cell. Ultra-LEAF™ purified anti-mouse GM-CSF recombinant (QA19A67) (purple squares) inhibits the effect of recombinant mouse GM-CSF at 0.3 ng/mL in FDC-P1 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.3 - 1.5 μg/mL
Cat # | Size | Price | Quantity Check Availability | Save | ||
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603053 | 100 µg | £245 | ||||
603054 | 1 mg | £825 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts, and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant mouse GM-CSF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Neut - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by inhibiting proliferation induced by recombinant mouse GM-CSF (Cat. No. 576306) at 0.3 ng/mL on FDC-P1 cells. ND50 range: 0.3 - 1.5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2922633 (BioLegend Cat. No. 603053)
AB_2922633 (BioLegend Cat. No. 603054)
Antigen Details
- Structure
- Cytokine
- Distribution
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T cells, Monocytes/Macrophages, Fibroblasts, Endothelial Cells, Mast Cells
- Function
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Interaction
- Granulocyte/Macrophage/Erythroid/Megakaryocytic Progenitors, Myeloblasts, Monoblasts
- Ligand/Receptor
- Heterodimer GM-CSFR µ subunit (CDw116), β-subunit (CDw131) in common with IL-3R, IL-5R
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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- Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press San Diego.
- Demetri G, et al. 1991. Blood. 78:2791-808.
- Fan D, et al. 1991. In vivo. 5:571-7.
- Negrin R, et al. 1992. Adv Pharmacol. 23:263-96.
- Gene ID
- 12981 View all products for this Gene ID
- UniProt
- View information about GM-CSF on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All GM-CSF Reagents Request Custom ConjugationDescription | Clone | Applications |
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Ultra-LEAF™ Purified anti-mouse GM-CSF Recombinant Antibody | QA19A67 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse GM-CSF Recombinant Antibody
Recombinant mouse GM-CSF (Cat. No. 576306) (black circles) i...
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