- Clone
- A15083D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1)
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15083D_ULEAF_CCL22_NEUT_Antibody_020717 A15083D_ULEAF_CCL22_NEUT_Antibody_020717](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15083D_ULEAF_CCL22_NEUT_Antibody_020717.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectants in a dose dependent manner (black squares). Anti-human CCL22 monoclonal antibody (purple circles) neutralizes the chemoattraction of Baf3-hCCR4 transfectants induced by recombinant human CCL22 (1.2 ng/ml). The ND50 = 0.4 - 2.4 µg/ml. -
Enriched human peripheral blood monocytes were cultured (3 days) with recombinant human GM-CSF. 1X Monensin (Cat. No. 420701) was added for the last 4 hours of culture. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and then intracellularly stained with Ultra-LEAF™ Purified anti-human CCL22 (clone A15083D) (left) or purified mouse IgG2a, κ isotype control (right) followed by anti-mouse IgG PE, then co-stained with anti-human CD206 (clone 15-2) APC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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694403 | 100 µg | £185 | ||||
694404 | 1 mg | £490 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CCL22 is thymus-specific chemokine engaged in the recruitment of T cells. CCL22 is associated with different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CCL22
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Neut - Quality tested
ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 1.5 - 4.5 µg/mL. For intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clones A15083A and A15083D do not block each other.
Clone A15083A does not block the reference clone T51-719 (BD Bioscience).
Clones A15083A and A15083D showed good staining in both Cyto-Fast™ Fix/Perm Buffer Set and True-Phos™ Perm Buffer. - RRID
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AB_2650729 (BioLegend Cat. No. 694403)
AB_2650730 (BioLegend Cat. No. 694404)
Antigen Details
- Structure
- Chemokine
- Distribution
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Macrophages, NK cells, IFN-γ stimulated human keratinocytes, thymic epithelial cells, and intestinal epithelial cells
- Function
- CCL22 induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFN-γ).
- Interaction
- Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
- Ligand/Receptor
- CCR4
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Godiska R, et al. 1997. J Exp Med. 185:1595-604.
- Imai T, et al. 1998. J Biol Chem. 273:16551-4.
- Yamashita U and Kuroda E. 2002. Crit Rev Immunol. 22:105-14.
- Horikawa T, et al. 2002. Int Immunol. 14:767-73.
- Mailloux AW and Young MR. 2009. J Immunol. 182:2753-65.
- Dogan RN, et al. 2011. J Leukoc Biol. 89:93-104.
- Osborn O, et al. 2011. Cytokine. 53:311-9.
- Gene ID
- 6367 View all products for this Gene ID
- UniProt
- View information about CCL22 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CCL22 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-human CCL22 | A15083D | Neut,ICFC |
Purified anti-human CCL22 | A15083D | Direct ELISA |
Biotin anti-human CCL22 | A15083D | Direct ELISA |
PE anti-human CCL22 | A15083D | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CCL22
Recombinant human CCL22 chemoattracts Baf3-hCCR4 transfectan... Enriched human peripheral blood monocytes were cultured (3 d... -
Purified anti-human CCL22
Direct ELISA showing purified anti-human CCL22 (clone A1508... -
Biotin anti-human CCL22
Direct ELISA showing biotin anti-human CCL22 (MDC) (clone A1... -
PE anti-human CCL22
Adherent peripheral blood mononuclear cells (PBMC) were cult...
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