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Regulatory T cells (Tregs) require IL-2 for their development, but it was unclear how IL-2 signaling impacted mature Tregs. To determine the role of IL-2 in mature Tregs, Fan et al. used an inducible knockdown mouse model to delete CD25, the ligand binding subunit of the IL-2 receptor, after thymic development. By performing adoptive transfer of cells with a CD25 knockdown or cells that had been sorted based on levels of CD25 expression, they determined that IL-2 signaling has unique functions in mature Tregs to promote proliferation and long-term survival and regulate glycolysis. Interestingly, in the absence of IL-2, Tregs persisted for several weeks in the presence of IL-7 indicating that IL-2 was not required to maintain lineage stability.

Adoptive transfer experiments can help to define how various signaling pathways affect cell development and function in vivo. We've released a new MojoSort™ CD45.1 magnetic cell separation kit to help isolate donor and recipient cells following adoptive transfer experiments utilizing CD45.1 and CD45.2 antigens.
Adapted from Fan, MY. et al. 2018. Cell Reports. 25(5):1204-13. Pubmed.
Specificity
Human CD19 Selection
Mouse CD45.1 Selection
Mouse Ly-6G Selection
Mouse P2RY12 Selection
MojoSort™ Mouse CD45.1 Selection Kit
A single cell suspension with a mix of approximately 10% SJL (CD45.1+) and 90% C57BL/6 (CD45.2+) splenocytes was selected using the Mouse CD45.1 Selection Kit. Cells were stained with anti-mouse CD45.1 (clone A20) and CD45.2 (clone 104).
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CD3 CD45.2
CD4 FOXP3
CD8 ICOS
CD25 IL-2
CD28 IL-7
CD45.1 PD-1
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