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| In flow cytometry, it is often imperative to study and isolate mononuclear cells (MNCs) from larger cell suspensions. BioLegend now offers Lymphopure™, a solution that is ready-to-use, filtered, and endotoxin-tested. The solution relies on the principle of density gradient centrifugation, which allows the separation of MNCs from other cells in blood such as granulocytes and erythrocytes. Granulocytes and erythrocytes will sediment after centrifugation. This convenient solution provides an easy method for MNC isolation for downstream applications like flow cytometry staining. |
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| Diagram showing whole blood cell separation using Lymphopure™. Blood is diluted with saline buffer and carefully layered over the medium in a conical tube, avoiding disturbing the interphase. Following centrifugation, at the appropriate speed with no braking, well-defined phases can be observed: 1-plasma, 2-interphase with mononuclear cells, 3-centrifugation medium, and 4-erythrocytes and granulocytes. Cells of interest can be aspirated from the interphase. |
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| Histogram overlay shows CD3 or CD15 profile on total cells from lysed peripheral human blood before (dotted histogram) and after Lymphopure™ (purple tinted histogram) or equivalent product (orange open histogram) centrifugation. CD3 is a molecule expressed by T cells, which are recovered with the use of the isolation media. CD15 is a molecule highly expressed by granulocytes, which are absent after isolation with the isolation media. |
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