CytoPhase™ Violet

Pricing & Availability
Other Names
live cell cycle, BrdU
Ave. Rating
0 reviews
CytoPhase_Violet_103116
Ramos cells treated with 5µM CytoPhase™ Violet dye for 90 minutes at 37°C. Cells were then acquired on a flow cytometer equipped with a 405 nm laser with a 450/50 bandpass filter.
  • CytoPhase_Violet_103116
    Ramos cells treated with 5µM CytoPhase™ Violet dye for 90 minutes at 37°C. Cells were then acquired on a flow cytometer equipped with a 405 nm laser with a 450/50 bandpass filter.
See Cytophase™ Violet spectral data
Description

CytoPhase™ Violet is a cell-permeant DNA-binding dye with the molecular weight of 560.96D and can be excited by either UV (355 nm or 375 nm) or violet laser (405 nm). Its emission maximum wavelength is about 440nm. It can be used for flow cytometric analysis of cell cycle in live or fixed cells. This dye can also be used for counterstaining the nuclei of cells in fluorescent microscopic imaging.

Product Details
Cat # Size Price Quantity Avail. Save
425701 200 µl $240.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Technical data sheet

Product Details

Formulation
5mM solution in H20 (200 µl/vial)
Concentration
5.0 mM
Storage & Handling
Upon receipt, CytoPhase™ Violet should be stored at 4°C and protected from light. When stored as recommended, the reagent is guaranteed for 1 year after purchase.
Application

FC - Quality tested

Recommended Usage

The recommended usage concentration for CytoPhase™ Violet is 5µM - 10µM.

Application Notes

Prepare cells of interest in appropriate cell culture media at a concentration of 0.5-1x 106 cells /ml. Add CytoPhase™ Violet dye to the cell suspension to bring it to 5µM (1:1000)-10µM (1:500) working solution and incubate for 90 minutes at 37°C (CO2 incubator). Optimal concentration might require titration of the dye based on differing cell types. Transfer cells to a FACS tube (cells do not require a wash step) and acquire samples on a flow cytometer equipped with a violet laser.

CytoPhase™ Violet can be used concurrently with antibody staining procedures. 

Antigen Details

Distribution
Nucleus, DNA.
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/31/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Request an Account