Flash Phalloidin™ Green 488

Pricing & Availability
Other Names
Phalloidin
Ave. Rating
0 reviews
Flash_Phalloidin_Green_488_IF_122916
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with anti-Cytokeratin Alexa Fluor® 647 (red) at 4°C overnight. The following day, the cells were stained with Flash Phalloidin™ Green 488 (green, 25 µL of the stock solution in 1 mL of PBS) for 30 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue). The image was captured by 40X objective.
  • Flash_Phalloidin_Green_488_IF_122916
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with anti-Cytokeratin Alexa Fluor® 647 (red) at 4°C overnight. The following day, the cells were stained with Flash Phalloidin™ Green 488 (green, 25 µL of the stock solution in 1 mL of PBS) for 30 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue). The image was captured by 40X objective.
  • Flash_Phalloidin_Green_488_IHC_122916
    C57BL/6 mouse frozen cerebellum section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature then permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/mL anti-GFAP (clone 2E1.E9) Alexa Fluor® 647 (red) at 4°C overnight. The following day the section was stained with Flash Phalloidin™ Green 488 (green, 25 µL of the stock solution
See Flash Phalloidin™ Green 488 spectral data See high resolution IF/IHC-F data...
Description

Molecular Mass 1582.65 g/mol-1.  Flash Phalloidin™ Green 488 excites maximally at 488 nm and emits maximally at 520 nm. 

Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom. This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which leads to cellular toxicity.  In cell imaging, this is a very useful probe for imaging and stabilizing filamentous F-actin in fixed and permeabilized cells, providing structural and volumetric context to the cell. Phallotoxins are conjugated to a wide array of fluorophores to enable their use in multicolor microscopy.

Product Details
Cat # Size Price Quantity Avail. Save
424201 300 units $295.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Technical data sheet

Product Details

Preparation
Flash Phalloidin™ Green 488 is lyophilized. Reconstitute with 1.5mL of methanol to make a stock solution of 300 units.
Storage & Handling
Store Flash Phalloidin™ Green 488 at -20°C, protected from light.
Application

IF - Quality tested
IHC-F - Validated

Recommended Usage

Reconstitute the Flash Phalloidin™ Green 488 with 1.5mL of methanol to make 300 units. Then prepare a working concentration by diluting 1:20 - 1:100 of stock in 1X PBS. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  1. Fix cultured cells with 1% - 4% paraformaldehyde (PFA) for 10 minutes at room temperature.
  2. Wash the cells two times with 1X PBS.
  3. Permeabilize the cells with 0.5% Triton X-100 for 10 minutes at room temperature or at 4°C.
  4. Wash the cells two times with 1X PBS.
  5. Block cells with 5% fetal bovine serum for 30 minutes at room temperature.
  6. Prepare the working solution by diluting Flash Phalloidin™ Green 488 1:20 - 1:100 in 1X PBS.
  7. Stain the cells with diluted solution for 20 minutes at room temperature in the dark protected from light.
  8. Mount the slides with an antifade mounting media and image the slides.

Antigen Details

Distribution

Cytoskeleton.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account