Human IL-31 ELISA MAX™ Deluxe

Pricing & Availability
Other Names
Interleukin-31, IL31 Precoated ELISA Kit
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  • Human_IL-31_ELISAMAX_Deluxe_122716

Interleukin 31 (IL-31) is a newly identified member of the gp130/IL-6 cytokine family that is composed of 144 amino acid and has molecular mass of 16 kD. IL-31 is produced mainly by T helper type 2 cells. The mature form of the protein contains a four-helix structure. Human IL-31 shares 31% amino acid sequence identity with mouse IL-31.

The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IL-31 specific goat polyclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-31 binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-human IL-31 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-31 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

Product Details
Cat # Size Price Quantity Avail. Save
445704 5 plates $295.00
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445705 10 Plates $450.00
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445706 20 Plates $665.00
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Technical data sheet

Kit Contents

Kit Contents
  • Human IL-31 ELISA MAX™ Capture Antibody (200X)
  • Human IL-31 ELISA MAX™ Detection Antibody (200X)
  • Human IL-31 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution C
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)
  • NUNC Maxisorp™ 96 MicroWell Plates
  • Lot-Specific Certificate of Analysis/ELISA MAX™ Deluxe Set Protocol

Product Details



As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Antigen Details

Cell Sources
Helper T Cell
Materials Not Included
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4,
    0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS +
    0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid
    solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper
0.6 pg/mL
Standard Range
37.5-2,400 pg/mL
Gene ID
386653 View all products for this Gene ID
View information about IL-31 on

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Go To Top Version: 1    Revision Date: 12/28/2016

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