LEGEND MAX™ Human IL-1α ELISA Kit with Pre-coated Plates

Pricing & Availability
Other Names
Interleukin-1α, LAF, EP, LEM, MCF/MNCF, IL-1α Precoated ELISA Kit
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LEGENDMAX_Human_IL-1alpha_ELISA
  • LEGENDMAX_Human_IL-1alpha_ELISA
Description

Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are from different gene products. IL-1α and IL-1β share about 25% amino acid sequence identity. They are initially synthesized as 31 kD precursors, and then cleaved by proteases into mature proteins with molecular mass approximately of 17 kD.

IL-1 binds to both forms of the cell surface type I (IL-1RI) and type II (IL-1RII) IL-1 receptors. IL-1RI is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes while IL-1RII is found on B-cell lineages, neutrophils, and bone marrow cells. Although both IL-1α and IL-1β are recognized by IL-1RI and IL-1RII receptors, IL-1α binds better to the type I receptor than IL-1β and vice versa.

IL-1 has different biological properties such as immunologic, pro-inflammatory, and protective effects. An increase of IL-1 by a variety of cells is a good indicator of infection by microbial toxins and inflammatory processes. It also affects B cell activation, immunocompetent cells and other immunologic cells. Studies show that the production of IL-1 has been detected in human joint fluids from patients with rheumatoid arthritis, osteoarthritis, traumatic arthritis, and psoriatic arthritis. In addition, IL-1α shows elevated levels in cancer such as colon cancer and cervical cancer.

The BioLegend LEGEND MAX™ Human IL-1α Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human IL-1α capture antibody. The detection antibody is a biotinylated goat polyclonal anti-human IL-1α antibody. This kit is specifically designed for the accurate quantitation of human IL-1α from cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Cat # Size Price Quantity Avail. Save
445807 1 Pre-coated Plate $350.00
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445808 5 Pre-coated Plates $1,250.00
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Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IL-1α Pre-coated 96-well Strip Microplate
  • Human IL-1α Dectection Antibody
  • Human IL-1α Standard
  • Avidin-HRP
  • Assay Buffer D
  • Matrix G (for EDTA plasma samples only)
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Antigen Details

Cell Sources
Macrophage
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials
Sensitivity
0.25 pg/mL ± 0.08 pg/mL
Standard Range
1.25-80 pg/mL
Gene ID
NA
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 12/28/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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