- Other Names
- Matrix Metalloproteinase 9, Gelatinase B, 92kD Type IV Collagenase, CLG4B
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Matrix metalloproteinase 9 (MMP-9), also known as gelatinase B or 92 kD type IV collagenase, belongs to the family of MMPs, which are zinc-dependent enzymes whose primary function is to degrade extracellular matrix proteins. MMP-9 is generally secreted as an inactive 92 kD pro-form by many cell types, notably neutrophils, monocytes, macrophages, endothelial cells, platelets, and tumor cells. A cysteine residue in the pro-domain coordinates with zinc in the catalytic domain to confer latency. When the pro-domain is cleaved off by other proteases, such as MMP-3, the enzyme is activated and forms its typical 82 kD mature form. An atypical 65 kD mature form also exists that lacks the hemopexin domain.
MMP-9 is the only MMP that can form homodimers, and both pro- and mature homodimer forms can exist. MMP-9 can also interact and bind to a multitude of other proteins. For example, tissue inhibitor of metalloproteinase 1 (TIMP-1) is its natural inhibitor. Other binding partners include CD44 and neutrophil gelatinase-associated lipocalin (NGAL).
MMP-9 is most closely related to MMP-2, with which it shares only 46% amino acid sequence identity overall. Across species, human MMP-9 shares 82-99%, 75%, and 72% amino acid sequence identity with non-human primate, rat, and mouse MMP-9, respectively.
MMP-9 plays important roles in inflammation, wound healing, tissue remodeling, cell migration, and tumor growth and metastasis. It is a potential biomarker for many diseases and conditions, often with elevated levels seen in various cancers, inflammatory conditions, allergies, autoimmune diseases, and myocardial infarction.
The LEGEND MAX™ Human MMP-9 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human MMP-9 antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human MMP-9 antibody. This kit is specifically designed to accurately measure MMP-9, including monomeric and dimeric pro-MMP-9, MMP-9/NGAL complex, and MMP-9/NGAL/TIMP-1 complex. This kit has been analytically validated to assess cell culture supernatant, urine, saliva, serum, and plasma samples with ready-to-use reagents.
|Cat #||Size||Price||Quantity Avail.||Save|
|444907||1 Pre-coated Plate||$395.00|
|444908||5 Pre-coated Plates||$1,395.00|
- Kit Contents
- Anti-human MMP-9 Pre-coated 96-well Strip Microplate
- Human MMP-9 Dectection Antibody
- Human MMP-9 Standard
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution D
- Stop Solution
- Plate Sealers
- Application Notes
This kit detects MMP-9 with better sensitivity than other kits on the market with the same diversity of valid sample types. The dynamic range of this kit is 0.25-16 ng/mL, while the dynamic range of an equivalent kit from a major competitor is 0.313-20 ng/mL. The overall sensitivity of this kit is 0.062 ng/mL soluble human MMP-9, while an equivalent kit from a major competitor has a sensitivity of 0.156 ng/mL.
- Cell Sources
- Neutrophils, monocytes, macrophages, endothelial cells, platelets, and tumor cells.
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- 46.9 pg/mL
- Standard Range
- 250-16,000 pg/mL
- Gene ID
- 4318 View all products for this Gene ID
- View information about MMP-9 on UniProt.org