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- Regulatory Status
- RUO
- Other Names
- Free TGF-β1 Assay, Total TGF-β1 Assay, Free Active TGF-β1 Assay
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | ||||
|---|---|---|---|---|---|---|
The LEGENDplex™ Free Active/Total TGF-β1 standard is a lyophilized standard for the Free Active/Total TGF-β1 Assay, which contains only TGF-β1 recombinant protein. This standard is recommended for use with:
740487 LEGENDplex™ Free Active/Toal TGF-β1 Detection Antibodies
740437 LEGENDplex™ Buffer Set E (Human) or 740491 LEGENDplex™ Buffer Set F (Mouse/Rat)
740488 LEGENDplex™ Free Active/Toal TGF-β1 Bead A6, 13X
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Application Notes
-
This standard is lyophilized from human TGF-ß1, but can be used for the Free Active/Total TGF-ß1 in multiple species. Buffer Set E (740437) used in human kit, Buffer Set F (740491) used in mouse/rat kit.
Antigen Details
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 7040 View all products for this Gene ID 21803 View all products for this Gene ID
- App Abbreviation (DOES NOT SHOW ON TDS):
- Multiplex - Component
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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