MojoSort™ Mouse NK Cell Isolation Kit

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MojoSort_Mouse_NK_Cell_Isolation_Kit_1_092716
A single cell suspension from C57BL/6 mouse spleen was prepared to isolate NK cells using the MojoSort™ Mouse NK cell Isolation Kit. Cells were stained with CD49b (clone DX5) PE and CD3 (clone 17A2) APC. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_NK_Cell_Isolation_Kit_1_092716
    A single cell suspension from C57BL/6 mouse spleen was prepared to isolate NK cells using the MojoSort™ Mouse NK cell Isolation Kit. Cells were stained with CD49b (clone DX5) PE and CD3 (clone 17A2) APC. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_NK_Cell_Isolation_Kit_2_092716
Description

Non-natural killer (NK) cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched NK cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Cat # Size Price Quantity Avail. Save
480049 20 tests $165.00
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480050 200 tests $495.00
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Technical data sheet

Kit Contents

Kit Contents

For Cat# 480049:

  • 200 µl of Biotin-Antibody Cocktail: Biotin anti- CD3, CD4, CD8b, CD24, Gr-1, I-A/I-E, CD19, TER-119/Erythroid, F4/80 and Ly-6C 
  • 200 µl Streptavidin Nanobeads

For Cat# 480050:

  • 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD3, CD4, CD8b, CD24, Gr-1, I-A/I-E, CD19, TER-119/Erythroid, F4/80 and Ly-6C
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched mouse NK cells from lymphoid tissues. The purity of the CD3-/CD49b+ NK Cell fraction typically ranges from 80 to 90%. Removal of dead cells is highly recommend prior to cell isolation. Dead cells can be removed using appropriate centrifugation media or other methods.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

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Go To Top Version: 1    Revision Date: 09/28/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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