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Purified anti-RBPJ Antibody

Pricing & Availability
Clone
W16038A (See other available formats)
Regulatory Status
RUO
Other Names
Recombining binding protein suppressor of hairless, CBF-1, Jκ-recombination signal-binding protein, IGKJRB, RBP-Jκ (RBPJK), Renal carcinoma antigen NY-REN-30, RBPSUH, recombination signal-binding protein Jκ
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16038A_PURE_RBPJ_Antibody_WB_021417
Total cell lysates (15µg protein) from MCF-7, HeLa, NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-RBPJ (clone W16038A) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. M*: protein standards with longer exposure time.
  • W16038A_PURE_RBPJ_Antibody_WB_021417
    Total cell lysates (15µg protein) from MCF-7, HeLa, NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-RBPJ (clone W16038A) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. M*: protein standards with longer exposure time.
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Description

Recombination signal-binding protein Jκ (RBPJ), also known as CBF-1, is a member of CSL family of transcription factors. RBPJ is the vertebrate ortholog of the Drosophila gene Suppressor of Hairless and is a highly conserved DNA-binding protein that mediates canonical Notch signaling. RBPJ is ubiquitously expressed and acts as a transcriptional repressor in the absence of Notch signaling. After ligand binding, Notch signaling is initiated by proteolytic cleavage and release of the active Notch intracellular domain (NICD). When it binds to the activated and nuclear-localized NICD, RBPJ recruits transcriptional coactivators and induces the activation of target genes containing consensus RBPJ-binding sequence C(T)GTGGGAA. RBPJ has been reported to be crucial in the cell fate determinations and regulation of cell proliferation and differentiation. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human RBPJ recombinant protein (101-350 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16038A does not cross-react with mouse (in-house tested).

Antigen Details

Structure
500 amino acids with a predicted molecular weight of 55.6 kD. Contains a IPT/TIG (Ig-like, plexins, transcription factors) domain that is involved in DNA-binding.
Distribution

Nucleus and cytoplasm.

Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations.
Interaction
Interacts with activated NOTCH1, NOTCH2, or NOTCH3. Interacts with HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2, CIR1, EP300, MAML1, PTF1A, EBNA2, EBNA3, EBNA4, EBNA6, RITA1/C12orf52, CHCHD2, CXXC5, BEND6, NKAPL.
Antigen References

1. Meyer Zu Horste G, et al. 2016. Cell Rep. 16:392.
2. Nielsen CM, et al. 2014. Development. 141:3782.
3. Zhang S, et al. 2014. Cell Res. 24:925.
4. Wang H, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:705.
5. Castel D, et al. 2013. Genes Dev. 27:1059.
6. Shi M, et al. 2012. J. Cell Sci. 125:4320.

Gene ID
3516 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
RBPJ
Specificity Alt (DOES NOT SHOW ON TDS):
RBPJ
App Abbreviation (DOES NOT SHOW ON TDS):
WB
UniProt
View information about RBPJ on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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