Recombinant Human GDF-6 (BMP-13) (carrier-free)

Pricing & Availability
Other Names
GDF6, BMP13, CDMP-2, Growth/differentiation factor 6, Bone morphogenetic protein 13, Cartilage-derived morphogenetic protein-2
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Human_GDF-6_CF_RECOM_1_111919_updated.png
Human GDF-6 induces alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5 with an EC50 of 0.05 - 0.2 µg/mL.
  • Human_GDF-6_CF_RECOM_1_111919_updated.png
    Human GDF-6 induces alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5 with an EC50 of 0.05 - 0.2 µg/mL.
  • Human_GDF-6_CF_RECOM_2_111919_updated.png
    Stability testing for human GDF-6. Recombinant human GDF-6 was aliquoted in 0.1% TFA at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in alkaline phosphatase production assay.
  • Human_GDF-6_CF_RECOM_3_111919_updated.png
    BioLegend's protein was compared side-by-side to a competitor’s equivalent product.
Cat # Size Price Quantity Avail. Save
788002 10 µg $70
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788004 25 µg $110
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788006 100 µg $275
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Description

Growth Differentiation Factor 6 (GDF-6, also known as BMP-13) is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptors, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptors and type II and IIB activin receptors. GDF-5, GDF-6 and GDF-7 define a subgroup within the BMP family. The mature form of GDF-6 is a 120 amino acid homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-6 was initially identified as a component of bovine cartilage. De novo implantation of GDF-6 induces neo-tendon/ligament formation in rats in connective tissue healing experiments. Mutation in the GDF6 gene is associated with Klippel Feil syndrome (KFS), characterised by congenital fusion of the cervical spine vertebrae, and causes defects in joint, ligament, and cartilage formation in a transgenic mouse model. Bone marrow multipotent mesenchymal stromal cells (BM MSCs) are capable of differentiating into multiple cell types including osteoblasts and chondrocytes. Using BM MSCs, it was identified that GDF-6 inhibits osteogenic differentiation, and therefore deceleration of calcium mineralization and reduction in alkaline phosphatase induction.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human GDF-6, amino acid Thr366-Arg455 (Accession # Q6KF10), was expressed in E.coli.
Molecular Mass
The 121 amino acid recombinant protein has a predicted molecular mass of approximately 13.7 kD. The DTT-reduced protein migrates at approximately 15 kD and and non-reduced protein migrates at approximately 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Purity
>95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in 0.1% TFA
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 = 0.4 - 2 µg/mL, as determined by induction of alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

Chondrocytes

Function
GDF-6 induces neo-tendon/ligament formation and inhibits osteogenic differentiation.
Interaction
Bone marrow multipotent mesenchymal stromal cells, chondroblasts
Ligand/Receptor
BMPR1A, BMPR1B, BMPR2 and ACVR2A
Bioactivity
Measured by its ability to induce alkaline phosphatase (ALP) production in the mouse chrondrogenic cell line ATDC5
Cell Type
Mesenchymal cells, Mesenchymal Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Stem Cells
Molecular Family
Growth Factors
Antigen References
  1. Chang SC, et al. 1994. J. Biol. Chem. 269:28227-34.
  2. Chen D, et al. 2004. Growth Factors. 22:233.
  3. Williams LA, et al. 2008. Int. J. Biol. Sci. 4:318.
  4. Tassabehji M, et al. 2008. Hum. Mutat. 29:1017.
  5. Shen B, et al. 2009. Int. J. Biol. Sci. 5:192.
Gene ID
392255 View all products for this Gene ID
UniProt
View information about GDF-6 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 11/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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