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Zombie Dyes

 

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It is critical to understand the degree of cell death in any flow cytometry assay and exclude those cells from the analysis. We provide DNA dyes, including Helix NP™ NIR, DRAQ7™, Propidium Iodide, and 7-AAD, that enter and stain dead cells, but are impermeable to live cells for rapid, cost-effective analysis of unfixed cells. In cases where cell fixation is required, we provide fixable Zombie Dyes. Not sure which Zombie is right for you? Try our Zombie Fixable Viability™ Sampler Kit, which contains 100 tests each of the UV, NIR, Violet, Aqua, and Yellow variants.

 

Zombie vs. DNA Dyes

Zombie Dyes are fixable dyes that react with primary amine groups on proteins. Live cells exclude that dyes, so that only cell surface proteins will be labeled, while dead cells allow the entry of the dyes into the cytoplasm increasing the amount of total protein labeling. Thus, dead cells will be significantly brighter for Zombie fluorescence than live cells.

DRAQ7™ , Propidium Iodide (PI), and 7-AAD (7-amino-actinomycin D) are high affinity DNA-binding dyes that are effectively excluded from live cells. Dead cells with compromised plasma membranes allow the passage of these dyes into the nucleus, where they will bind DNA. These dyes produce a bright fluorescent signal when excited by the appropriate lasers.

Zombie Dyes by Excitation Laser

Click the plus next to each excitation laser to see the available Zombie Dyes and their corresponding spectra.
Or, examine them on our spectra analyzer tools.

(+)    UV Laser

 

Zombie UV™ is excited by the UV laser (350 nm) and emits maximally at 459 nm.

(+)    Violet Laser

 

Zombie Violet™ is excited by the violet laser (405 nm) and emits maximally at 423 nm. It has similar emission to Brilliant Violet 421™ and Pacific Blue™.

Zombie Aqua™ is excited by the violet laser (405 nm) and the ultraviolet laser (350 nm). It emits maximally at 516 nm.

Zombie Yellow™ is excited by the violet laser (405 nm) and partially by the UV laser (350 nm). It emits maximally at 572 nm and has a similar emission profile to Brilliant Violet 570™.

(+)    Blue Laser

 

Zombie Green™ is excited by the blue laser (488 nm) and emits maximally at 515 nm.

Zombie B550™ is excited by the blue laser (488 nm) and emits maximally at 540 nm. It has similar emission spectra to Spark Blue™ 550.

 

Zombie YG™ 581 is excited by the yellow/green laser (561 nm) and emits maximally at 581 nm. It has similar emission spectra to Spark YG™ 581.

Zombie Red™ is excited by the yellow/green laser (561 nm) and emits maximally at 624 nm. Zombie Red can be weakly excited by the blue laser (488 nm) if a yellow/green laser is not available. This may require a higher concentration of dye to be used along with optimization by the end user.

(+)    Red Laser

 

Zombie R685™ is excited by the red laser (633 nm) and emits maximally at 685 nm. It has similar emission spectra to Spark NIR™ 685.

Zombie R718™ is excited by the red laser (633 nm) and emits maximally at 718 nm. It has similar emission spectra to Spark Red™ 718.

Zombie NIR™ is excited by the red laser (633 nm) and emits maximally at 746 nm.

Name Size Catalog No.
Zombie UV™ Fixable Viability Kit 100 tests | 500 tests 423107 | 423108
Zombie Violet™ Fixable Viability Kit 100 tests | 500 tests 423113 | 423114
Zombie Aqua™ Fixable Viability Kit 100 tests | 500 tests 423101 | 423102
Zombie Yellow™ Fixable Viability Kit 100 tests | 500 tests 423103 | 423104
Zombie Green™ Fixable Viability Kit 100 tests | 500 tests 423111 | 423112
Zombie B550™ Fixable Viability Kit 100 tests | 500 tests 423121 | 423122
Zombie YG581™ Fixable Viability Kit 100 tests | 500 tests 423123 | 423124
Zombie Red™ Fixable Viability Kit 100 tests | 500 tests 423109 | 423110
Zombie R685™ Fixable Viability Kit 100 tests | 500 tests 423119 | 423120
Zombie R718™ Fixable Viability Kit 100 tests | 500 tests 423115 | 423116
Zombie NIR™ Fixable Viability Kit 100 tests | 500 tests 423105 | 423106
Zombie Fixable Viability™ Sampler Kit 1 kit (500 tests total) 423117

Zombie Staining Strategy

 

Plan/Strategy

Available Options

I plan to run my cells live through the flow cytometer without any fixation (sorting live cells).

Propidium Iodide, 7-AAD, or Zombie dyes.

I plan to fix my cells before I do any staining with antibodies.

Zombie Dyes prior to fixation.

I plan to stain my cells with antibodies and then fix my cells.

Zombie Dyes prior to antibody staining and fixation.

I plan to fix and permeabilize my cells for intercellular antibody staining.

Zombie Dyes prior to fixation.

My cells are already fixed.

No Live/Dead options available.
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