Alexa Fluor® 647 anti-human TRA-2-54 Antibody

Pricing & Availability
Clone
TRA-2-54/2J (See other available formats)
Other Names
Liver/Bone/Kidney Alkaline Phosphatase
Isotype
Mouse IgG1, κ
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Product Citations
publications
TRA-2-54-2J_A647_TRA-2-54_Antibody_ICC_060619
Human stem cell line NCCIT was fixed with 1% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes. Then, the cells were stained with 10 µg/ml of anti-human TRA-2-54 (clone TRA-2-54/2J) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
  • TRA-2-54-2J_A647_TRA-2-54_Antibody_ICC_060619
    Human stem cell line NCCIT was fixed with 1% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes. Then, the cells were stained with 10 µg/ml of anti-human TRA-2-54 (clone TRA-2-54/2J) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
358810 100 µg $325
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Description

TRA-2-54, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone and kidney cells. It has been reported that the expression level of TRA-2-54 is decreased upon differentiation; therefore measurement of TRA-2-54 expression can be used to monitor the differentiation status of pluripotent stem cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonal carcinoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemistry1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Zou XY, et al. 2012. Stem Cell Res. Ther. 3: 43. (IHC, IF)
RRID
AB_2616775 (BioLegend Cat. No. 358810)

Antigen Details

Distribution

Undifferentiated embryonic stem (ES) cells, embryonal carcinoma (EC) cells, most EC cell lines, bone, liver, kidney

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Andrews PW, et al. 1996. Int. J. Cancer. 66:806. 
2. Yan X, et al. 2010. Stem Cells Dev. 19:469. 
3. Zou XY, et al. 2012. Stem Cell Res Ther. 3:43.

Gene ID
249 View all products for this Gene ID
UniProt
View information about TRA-2-54 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/13/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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