Biotin anti-mouse CD123 Antibody

Pricing & Availability
Clone
5B11 (See other available formats)
Other Names
IL-3 Receptor α chain, IL-3Rα
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_5B11_Biotin_082109.jpg
C57BL/6 bone marrow cells double stained with CD11b FITC and biotinylated 5B11, followed by Sav-PE
  • A_5B11_Biotin_082109.jpg
    C57BL/6 bone marrow cells double stained with CD11b FITC and biotinylated 5B11, followed by Sav-PE
  • B_5B11_Biotin_082109.jpg
    C57BL/6 bone marrow cells double stained with rat IgG2a (Clone RTK2758) biotin isotype control followed by Sav-PE and CD11b (Clone M1/70) FITC
Cat # Size Price Quantity Avail. Save
106003 50 µg $75
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Description

CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CTLL cells transfected with the mouse IL-3Rα chain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.

Application References

(PubMed link indicates BioLegend citation)
  1. Ichihara, et al. 1995. EMBO J. 14:939. (FC)
  2. Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC)
  3. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)
Product Citations
  1. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
RRID
AB_2124404 (BioLegend Cat. No. 106003)

Antigen Details

Structure
Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD
Distribution

Hematopoietic progenitors, basophils, mast cells, megakaryocytes

Function
Proliferation, differentiation of hematopoietic cells
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Miyajima A, et al. 1993. Blood 82:1960.
3. Itoh N, et al. 1990. Science 247:324.

Gene ID
16188 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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