Boost DAB Enhancer, 50X (Previously Covance catalog# SIG-31047)

Pricing & Availability
Other Names
DAB Enhancer Solution
Previously
Signet Catalog# 1047
Covance Catalog# SIG-31047
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Cat # Size Price Quantity Avail. Save
926701 3 ml $44.00
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Technical data sheet

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

Protocol:
1. Warm the Boost DAB Enhancer to room temperature before use.
2. Add the DAB chromogen, DAB substrate buffer and Boost DAB Enhancer as described below.
3. Apply mixture to tissue section 
4. Incubate the slides for 5-10 min. at room temperature
5. Rinse tissue sections with deionized water
6. Counterstain and mount
7. DAB produces a brown precipitate with almost no background

Application Notes

BioLegend’s Boost DAB Enhancer consists of a metal based solution that when used in conjunction with the DAB chromogen produces a clear brown - black precipitate that is insoluble in alcohol. The slides may be dehydrated through alcohols to xylene and mounted with any commercially available mounting media.

Prepare the DAB solution by combining components as described in the following table.

DAB        DAB              DAB
Buffer    Chromogen    Boost Enhancer
  5 mL    +   0.2 mL   +   0.1 mL
25 mL   +   1.0 mL   +   0.5 mL
50 mL   +   2.0 mL   +   1.0 mL

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 03/27/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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