DAB Substrate Buffer

Pricing & Availability
Other Names
Substrate Buffer
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Description

In the presence of peroxidase enzyme, DAB will produce a brown precipitate that is insoluble in alcohol. This product is the substrate buffer for use with DAB chromogen (Cat. Nos. 926506 and 926507).

Product Details
Cat # Size Price Quantity Avail. Save
926605 15 ml $45.00
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926606 1 L $357.00
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Technical data sheet

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

Each lot of this product is quality tested by immunohistochemistry.

Prepare the DAB solution by combining components as described in the following table.

Buffer     DAB Chromogen
5 mL   +   0.2 mL
25 mL +   1.0 mL
50 mL +   2.0 mL

Application Notes

Protocol:
1. Add the DAB chromogen to the DAB substrate buffer as described above
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 minutes at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 0    Revision Date: 03/04/2016

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